Effect of St John's wort (Hypericum perforatum) on cytochrome P-450 activity in perfused rat liver

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F05%3A00013572" target="_blank" >RIV/60461373:22310/05:00013572 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/05:00014108

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of St John's wort (Hypericum perforatum) on cytochrome P-450 activity in perfused rat liver

Popis výsledku v původním jazyce

St. John's wort (Hypericum perforatum) is a popular over-the-counter dietary supplement and a herbal antidepressant that has been implicated in drug interactions with substrates of several cytochrome P-450 (CYP) isozymes. The effects of the St. John's wort extract (100 mg/kg, i.p., once daily for 10 days) on metabolic activity of CYP450 were assessed in the system of isolated perfused rat liver. The substrates used in this study were tolbutamide (CYP2C6), dextromethorphan (CYP2D2) and midazolam (CYP3A2). Validated HPLC method was used to quantify all compounds of interest. St. John's wort administration affected CYP activity, causing a significant decline in AUC of dextromethorphan [F(4,31) = 1511, p < 0.001; PLSD, p < 0.001] and AUC of midazolam [F(3,25) = 221, p < 0.001; PLSD, p = 0.035] and a significant increase in AUC of tolbutamide [F(3,26) = 200, p < 0.001; PLSD, p < 0.001]. St. John's wort administration resulted in a significant induction of CYP2D2 and CYP3A

Název v anglickém jazyce

Effect of St John's wort (Hypericum perforatum) on cytochrome P-450 activity in perfused rat liver

Popis výsledku anglicky

St. John's wort (Hypericum perforatum) is a popular over-the-counter dietary supplement and a herbal antidepressant that has been implicated in drug interactions with substrates of several cytochrome P-450 (CYP) isozymes. The effects of the St. John's wort extract (100 mg/kg, i.p., once daily for 10 days) on metabolic activity of CYP450 were assessed in the system of isolated perfused rat liver. The substrates used in this study were tolbutamide (CYP2C6), dextromethorphan (CYP2D2) and midazolam (CYP3A2). Validated HPLC method was used to quantify all compounds of interest. St. John's wort administration affected CYP activity, causing a significant decline in AUC of dextromethorphan [F(4,31) = 1511, p < 0.001; PLSD, p < 0.001] and AUC of midazolam [F(3,25) = 221, p < 0.001; PLSD, p = 0.035] and a significant increase in AUC of tolbutamide [F(3,26) = 200, p < 0.001; PLSD, p < 0.001]. St. John's wort administration resulted in a significant induction of CYP2D2 and CYP3A

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Life Sciences

ISSN

0024-3205

e-ISSN

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Svazek periodika

78

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

239-244

Kód UT WoS článku

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EID výsledku v databázi Scopus

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