HPLC Method for the Determination of the Purity of K[Pt(NH3)Cl3], a Precursor of the Platinum Complexes with Cytostatic Activity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F11%3A43891933" target="_blank" >RIV/60461373:22310/11:43891933 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/00032719.2010.546027" target="_blank" >http://dx.doi.org/10.1080/00032719.2010.546027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/00032719.2010.546027" target="_blank" >10.1080/00032719.2010.546027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

HPLC Method for the Determination of the Purity of K[Pt(NH3)Cl3], a Precursor of the Platinum Complexes with Cytostatic Activity

Popis výsledku v původním jazyce

K[Pt(NH3)Cl3], a valuable precursor for the prepn. of platinum complexes with cytostatic activity, e.g. satraplatin, picoplatin, LA-12, and cycloplatam, is currently prepd. from cis-[Pt(NH3)2Cl2] or K2[PtCl4] and these are the usual impurities in the final product. A simple, selective, and sensitive HPLC-UV anal. method for the detn. of the purity of K[Pt(NH3)Cl3] and the quantification of the impurities was developed and validated. The platinum complexes present in the final product were sepd. on a strong base ion exchange column by the gradient elution with detection at 213 nm. Intra-assay precisions for the platinum complexes resp. to their ions ([PtCl4]2-, [Pt(NH3)Cl3]-, and cis-[Pt(NH3)2Cl2]) were between 0.1% and 2.0% (relative std. deviation); intermediate precisions were between 1.4% and 2.0% and accuracies were between 98.6% and 101.4%. Limits of detection of [PtCl4]2-, [Pt(NH3)Cl3]-, and cis-[Pt(NH3)2Cl2] were 6 mýg/mL-1, 13 mg/mL-1, and 5 mýg/mL-1 resp., limits of quantifica

Název v anglickém jazyce

HPLC Method for the Determination of the Purity of K[Pt(NH3)Cl3], a Precursor of the Platinum Complexes with Cytostatic Activity

Popis výsledku anglicky

K[Pt(NH3)Cl3], a valuable precursor for the prepn. of platinum complexes with cytostatic activity, e.g. satraplatin, picoplatin, LA-12, and cycloplatam, is currently prepd. from cis-[Pt(NH3)2Cl2] or K2[PtCl4] and these are the usual impurities in the final product. A simple, selective, and sensitive HPLC-UV anal. method for the detn. of the purity of K[Pt(NH3)Cl3] and the quantification of the impurities was developed and validated. The platinum complexes present in the final product were sepd. on a strong base ion exchange column by the gradient elution with detection at 213 nm. Intra-assay precisions for the platinum complexes resp. to their ions ([PtCl4]2-, [Pt(NH3)Cl3]-, and cis-[Pt(NH3)2Cl2]) were between 0.1% and 2.0% (relative std. deviation); intermediate precisions were between 1.4% and 2.0% and accuracies were between 98.6% and 101.4%. Limits of detection of [PtCl4]2-, [Pt(NH3)Cl3]-, and cis-[Pt(NH3)2Cl2] were 6 mýg/mL-1, 13 mg/mL-1, and 5 mýg/mL-1 resp., limits of quantifica

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anal.Lett.

ISSN

0003-2719

e-ISSN

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Svazek periodika

44

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

2182-2193

Kód UT WoS článku

000294602900011

EID výsledku v databázi Scopus

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