Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography-tandem mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F11%3A43892008" target="_blank" >RIV/60461373:22310/11:43892008 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023752:_____/11:00001182 RIV/00216208:11120/11:00003215

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2011.02.006" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2011.02.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2011.02.006" target="_blank" >10.1016/j.chroma.2011.02.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography-tandem mass spectrometry

Popis výsledku v původním jazyce

A sensitive assay method was developed for a parallel, rapid and precise detn. of dopamine and its metabolites, homovanillic acid, 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid, from brain microdialyzates. The method consisted of a pretreatment step, freeze-drying (lyophilization), to conc. dopamine and its metabolites from the microdialyzates, and a detection step using liq. chromatog. combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, the reaction monitoring mode was selected for its extremely high degree of selectivity and the stable-isotope-diln. assay for its high precision of quantification. The developed method was characterized by the following parameters: the precision of the developed method was detd. as ?88.6% for dopamine, ?89.9% for homovanillic acid, ?86.1% for 3-methoxytyramine and ?88.1% for 3,4-dihydroxyphenylacetic acid; the mean accuracy was detd. as ?88.2% for dopamine, ?88.3% for homovanillic acid, ?85.9% for 3-meth

Název v anglickém jazyce

Monitoring of dopamine and its metabolites in brain microdialysates: Method combining freeze-drying with liquid chromatography-tandem mass spectrometry

Popis výsledku anglicky

A sensitive assay method was developed for a parallel, rapid and precise detn. of dopamine and its metabolites, homovanillic acid, 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid, from brain microdialyzates. The method consisted of a pretreatment step, freeze-drying (lyophilization), to conc. dopamine and its metabolites from the microdialyzates, and a detection step using liq. chromatog. combined with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). In particular, the reaction monitoring mode was selected for its extremely high degree of selectivity and the stable-isotope-diln. assay for its high precision of quantification. The developed method was characterized by the following parameters: the precision of the developed method was detd. as ?88.6% for dopamine, ?89.9% for homovanillic acid, ?86.1% for 3-methoxytyramine and ?88.1% for 3,4-dihydroxyphenylacetic acid; the mean accuracy was detd. as ?88.2% for dopamine, ?88.3% for homovanillic acid, ?85.9% for 3-meth

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1218

Číslo periodika v rámci svazku

21

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

3382-3391

Kód UT WoS článku

000290883000036

EID výsledku v databázi Scopus

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