Carcinogenic 3-nitrobenzanthrone but not 2-nitrobenzanthrone is metabolised to an unusual mercapturic acid in rat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F12%3A43891654" target="_blank" >RIV/60461373:22310/12:43891654 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.toxlet.2011.11.017" target="_blank" >http://dx.doi.org/10.1016/j.toxlet.2011.11.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxlet.2011.11.017" target="_blank" >10.1016/j.toxlet.2011.11.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carcinogenic 3-nitrobenzanthrone but not 2-nitrobenzanthrone is metabolised to an unusual mercapturic acid in rat

Popis výsledku v původním jazyce

3-Nitrobenzanthrone (3-NBA) is an extremely potent mutagen and suspect human carcinogen found in diesel exhaust. Its isomer 2-nitrobenzanthrone (2-NBA) has also been found in ambient air. These isomers differ in mutagenicity in Salmonella by 2 - 3 ordersof magnitude. To identify their urinary metabolites and also to assess the assumed differences in their excretion, rats were dosed orally with 2 mg/kg b.w. of either 2-NBA or 3-NBA. Both LC-ESI-MS and GC-MS confirmed formation of the corresponding aminobenzanthrones (ABA). A novel mercapturic acid metabolite of 3-NBA was identified in urine by LC-ESI-MS as N-acetyl-S-(3-aminobenzanthron-2-yl)cysteine (3-ABA-MA) by comparison with the authentic standard. Its excretion amounted to 0.49 +/- 0.15 and 0.02+/- 0.01 % of dose within the first and second day after dosing, respectively. In contrast, no mercapturic acid was detected in the urine of rats dosed with 2-NBA.

Název v anglickém jazyce

Carcinogenic 3-nitrobenzanthrone but not 2-nitrobenzanthrone is metabolised to an unusual mercapturic acid in rat

Popis výsledku anglicky

3-Nitrobenzanthrone (3-NBA) is an extremely potent mutagen and suspect human carcinogen found in diesel exhaust. Its isomer 2-nitrobenzanthrone (2-NBA) has also been found in ambient air. These isomers differ in mutagenicity in Salmonella by 2 - 3 ordersof magnitude. To identify their urinary metabolites and also to assess the assumed differences in their excretion, rats were dosed orally with 2 mg/kg b.w. of either 2-NBA or 3-NBA. Both LC-ESI-MS and GC-MS confirmed formation of the corresponding aminobenzanthrones (ABA). A novel mercapturic acid metabolite of 3-NBA was identified in urine by LC-ESI-MS as N-acetyl-S-(3-aminobenzanthron-2-yl)cysteine (3-ABA-MA) by comparison with the authentic standard. Its excretion amounted to 0.49 +/- 0.15 and 0.02+/- 0.01 % of dose within the first and second day after dosing, respectively. In contrast, no mercapturic acid was detected in the urine of rats dosed with 2-NBA.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/2B08051" target="_blank" >2B08051: Nová strategie biologického monitorování exposice mutagenům a karcinogenům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Svazek periodika

208

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

7

Strana od-do

246-253

Kód UT WoS článku

000301018100006

EID výsledku v databázi Scopus

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