The development of a sensitive immunoassay for the detection of new synthetic drugs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F13%3A43903221" target="_blank" >RIV/60461373:22310/13:43903221 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/13:43903221

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The development of a sensitive immunoassay for the detection of new synthetic drugs

Popis výsledku v původním jazyce

New synthetic psychoactive drugs are the most destructive and available compounds today. Older drugs as heroin or cocaine destroy person's life but new drugs are more dangerous because these compounds not only cause overdose, a psychotic episode of hallucinations, aggression, impulses and homicidal tendencies. Using of these drugs is widening amongst young people. Consequently, there is growing public interest in development of versatile detection methods enabling to effectively intervene in this field. Traditional methods of analysis of narcotics are gas and liquid chromatography - GC/MS, LC/MS. Their advantages are high sensitivity and reliability. On the contrary, instrumental methods are less rapid and they involve multiple steps in sample preparation and analysis, require expensive equipment and skilled analysts, and therefore they are unsuitable for routine analysis of a large number of samples or on-site determinations. An alternative approach is represented by immunoassays which could be portable and cost-effective, with adequate sensitivity, high selectivity and a simple sample processing. In this work, enzyme -linked imunnosorbent assays have been developed for the detection of selected synthetic cathinones. Carboxylated derivatives of parent drugs were prepared as haptens for synthesis of immunogens and subsequently a series of polyclonal rabbit antibodies were prepared. The antibodies and haptens were used as immunoreagents in competitive Enzyme-linked immunosorbent assay. Under optimal experimental conditions, the most sensitive assay achieved IC50 and limit of detection values of 2 ng/ml and 0.09 ng/mL, respectively. We conclude that after the validation for individual matrices, these methods enable effective fast detection of synthetic cathinones in operational conditions.

Název v anglickém jazyce

The development of a sensitive immunoassay for the detection of new synthetic drugs

Popis výsledku anglicky

New synthetic psychoactive drugs are the most destructive and available compounds today. Older drugs as heroin or cocaine destroy person's life but new drugs are more dangerous because these compounds not only cause overdose, a psychotic episode of hallucinations, aggression, impulses and homicidal tendencies. Using of these drugs is widening amongst young people. Consequently, there is growing public interest in development of versatile detection methods enabling to effectively intervene in this field. Traditional methods of analysis of narcotics are gas and liquid chromatography - GC/MS, LC/MS. Their advantages are high sensitivity and reliability. On the contrary, instrumental methods are less rapid and they involve multiple steps in sample preparation and analysis, require expensive equipment and skilled analysts, and therefore they are unsuitable for routine analysis of a large number of samples or on-site determinations. An alternative approach is represented by immunoassays which could be portable and cost-effective, with adequate sensitivity, high selectivity and a simple sample processing. In this work, enzyme -linked imunnosorbent assays have been developed for the detection of selected synthetic cathinones. Carboxylated derivatives of parent drugs were prepared as haptens for synthesis of immunogens and subsequently a series of polyclonal rabbit antibodies were prepared. The antibodies and haptens were used as immunoreagents in competitive Enzyme-linked immunosorbent assay. Under optimal experimental conditions, the most sensitive assay achieved IC50 and limit of detection values of 2 ng/ml and 0.09 ng/mL, respectively. We conclude that after the validation for individual matrices, these methods enable effective fast detection of synthetic cathinones in operational conditions.

Druh

O - Ostatní výsledky

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/VG20122015075" target="_blank" >VG20122015075: Nové syntetické drogy (NSD) - vytvoření komplexní toxikologické databáze, vývoj metodik jejich detekce včetně rychlých imunochemických testů, jejich behaviorální farmakologie, farmakokinetika a biotransformace u potkanů, epidemiologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů