Structure, mechanical characteristics and in vitro degradation, cytotoxicity, genotoxicity and mutagenicity of novel biodegradable Zn-Mg alloys

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F16%3A43900033" target="_blank" >RIV/60461373:22310/16:43900033 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.msec.2015.08.015" target="_blank" >http://dx.doi.org/10.1016/j.msec.2015.08.015</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2015.08.015" target="_blank" >10.1016/j.msec.2015.08.015</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure, mechanical characteristics and in vitro degradation, cytotoxicity, genotoxicity and mutagenicity of novel biodegradable Zn-Mg alloys

Popis výsledku v původním jazyce

Zn-(0-1.6)Mg (in wt.%) alloys were prepared by hot extrusion at 300 degrees C. The structure, mechanical properties and in vitro biocompatibility of the alloys were investigated. The hot-extruded magnesium-based WE43 alloy was used as a control. Mechanical properties were evaluated by hardness, compressive and tensile testing. The cytotoxicity, genotoxicity (comet assay) and mutagenicity (Ames test) of the alloy extracts and ZnCl2 solutions were evaluated with the use of murine fibroblasts L929 and human osteosarcoma cell line U-2 OS. The microstructure of the Zn alloys consisted of recrystallized Zn grains of 12 11111 in size and fine Mg2Zn1.1 particles arranged parallel to the hot extrusion direction. Mechanical tests revealed that the hardness and strength increased with increasing Mg concentration. The Zn-0.8 Mg alloys showed the best combination of tensile mechanical properties (tensile yield strength of 203 MPa, ultimate tensile strength of 301 MPa and elongation of 15%). At higher Mg concentrations the plasticity of Zn-Mg alloys was deteriorated. Cytotoxicity tests with alloy extracts and ZnCl2 solutions proved the maximum safe Zn2+ concentrations of 120 mu M and 80 mu M for the 0-2 OS and L929 cell lines, respectively. Ames test with extracts of alloys indicated that the extracts were not mutagenic. The comet assay demonstrated that 1-day extracts of alloys were not genotoxic for U-2 OS and L929 cell lines after 1-day incubation.

Název v anglickém jazyce

Structure, mechanical characteristics and in vitro degradation, cytotoxicity, genotoxicity and mutagenicity of novel biodegradable Zn-Mg alloys

Popis výsledku anglicky

Zn-(0-1.6)Mg (in wt.%) alloys were prepared by hot extrusion at 300 degrees C. The structure, mechanical properties and in vitro biocompatibility of the alloys were investigated. The hot-extruded magnesium-based WE43 alloy was used as a control. Mechanical properties were evaluated by hardness, compressive and tensile testing. The cytotoxicity, genotoxicity (comet assay) and mutagenicity (Ames test) of the alloy extracts and ZnCl2 solutions were evaluated with the use of murine fibroblasts L929 and human osteosarcoma cell line U-2 OS. The microstructure of the Zn alloys consisted of recrystallized Zn grains of 12 11111 in size and fine Mg2Zn1.1 particles arranged parallel to the hot extrusion direction. Mechanical tests revealed that the hardness and strength increased with increasing Mg concentration. The Zn-0.8 Mg alloys showed the best combination of tensile mechanical properties (tensile yield strength of 203 MPa, ultimate tensile strength of 301 MPa and elongation of 15%). At higher Mg concentrations the plasticity of Zn-Mg alloys was deteriorated. Cytotoxicity tests with alloy extracts and ZnCl2 solutions proved the maximum safe Zn2+ concentrations of 120 mu M and 80 mu M for the 0-2 OS and L929 cell lines, respectively. Ames test with extracts of alloys indicated that the extracts were not mutagenic. The comet assay demonstrated that 1-day extracts of alloys were not genotoxic for U-2 OS and L929 cell lines after 1-day incubation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JG - Hutnictví, kovové materiály

OECD FORD obor

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Projekt

<a href="/cs/project/GBP108%2F12%2FG043" target="_blank" >GBP108/12/G043: Mikro- a nanokrystalické materiály s vysokým podílem rozhraní pro moderní strukturní aplikace, biodegradabilní implantáty a uchovávání vodíku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials science and engineering C

ISSN

0928-4931

e-ISSN

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Svazek periodika

neuveden

Číslo periodika v rámci svazku

28

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

24-35

Kód UT WoS článku

000364247500004

EID výsledku v databázi Scopus

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