DEVELOPMENT OF ANTIBACTERIAL ACTIVITY TESTING METHOD

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F16%3A43903574" target="_blank" >RIV/60461373:22310/16:43903574 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/16:43903574

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

DEVELOPMENT OF ANTIBACTERIAL ACTIVITY TESTING METHOD

Popis výsledku v původním jazyce

Titanium and its alloys are widely used metallic materials in the construction of orthopaedic and dental implants. Surface condition has significant impact on the interaction between living tissue and implant. One of the most important demands is to create a bioactive surface. It can be achieved e.g. by anodic oxidation. This modification can result in highly ordered nanostructure, which can be suitably modified. Colonization of implanted biomaterial by bone cells may be affected by undesirable processes. One of them is bacterial attack resulting in infection which is one of the reasons leading to implant failure.Silver is well known for its strong anti-bacterial activity against wide range of micro-organisms. Silver was deposited on the surface of nanostructured samples by various methods - PVD, photochemical reduction, electrochemical deposition, sol-gel method - in the frame of this work. The main aim of the study was to develop a method for precise assessment of flat samples surface antibacterial activity. Antibacterial efficacy was tested using the indicator strains of bacteria Escherichia Coli and Staphylococcus Aureus. Bacterial culture was applied to the sample surface. After appropriate treatment surviving bacteria were cultivated on Agar and after desirable period colonies were evaluated quantitatively. Method for antibacterial properties testing of flat surfaces was developed. The effectiveness of silver has been confirmed in all cases. Surprisingly even unmodified nanostructure shown anti-bacterial activity to some extent. Based on results Staphylococcus Aureus is more stable species and will be used in subsequent tests.

Název v anglickém jazyce

DEVELOPMENT OF ANTIBACTERIAL ACTIVITY TESTING METHOD

Popis výsledku anglicky

Titanium and its alloys are widely used metallic materials in the construction of orthopaedic and dental implants. Surface condition has significant impact on the interaction between living tissue and implant. One of the most important demands is to create a bioactive surface. It can be achieved e.g. by anodic oxidation. This modification can result in highly ordered nanostructure, which can be suitably modified. Colonization of implanted biomaterial by bone cells may be affected by undesirable processes. One of them is bacterial attack resulting in infection which is one of the reasons leading to implant failure.Silver is well known for its strong anti-bacterial activity against wide range of micro-organisms. Silver was deposited on the surface of nanostructured samples by various methods - PVD, photochemical reduction, electrochemical deposition, sol-gel method - in the frame of this work. The main aim of the study was to develop a method for precise assessment of flat samples surface antibacterial activity. Antibacterial efficacy was tested using the indicator strains of bacteria Escherichia Coli and Staphylococcus Aureus. Bacterial culture was applied to the sample surface. After appropriate treatment surviving bacteria were cultivated on Agar and after desirable period colonies were evaluated quantitatively. Method for antibacterial properties testing of flat surfaces was developed. The effectiveness of silver has been confirmed in all cases. Surprisingly even unmodified nanostructure shown anti-bacterial activity to some extent. Based on results Staphylococcus Aureus is more stable species and will be used in subsequent tests.

Druh

O - Ostatní výsledky

CEP obor

JG - Hutnictví, kovové materiály

OECD FORD obor

—

Projekt

<a href="/cs/project/TE01020390" target="_blank" >TE01020390: Centrum vývoje moderních kovových biomateriálů pro lékařské implantáty</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů