PEGylated gold nanoparticles: Stability, cytotoxicity and antibacterial activity
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918406" target="_blank" >RIV/60461373:22310/19:43918406 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/44555601:13440/19:43894735 RIV/00216208:11110/19:10383266 RIV/00216208:11140/19:10383266 RIV/00216208:11320/19:10383266
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0927775718312299?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0927775718312299?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.colsurfa.2018.09.083" target="_blank" >10.1016/j.colsurfa.2018.09.083</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
PEGylated gold nanoparticles: Stability, cytotoxicity and antibacterial activity
Popis výsledku v původním jazyce
Designing safe nanomaterials is nowadays a critical issue. A way to prepare PEGylated Au NPs by sputtering of Au into pure (PEG), thiol (PEG-SH) and amine terminated polyethylene glycols (PEG-NH2) was explored. Structural characterizations, aging and thermal stability were performed using transmission electron microscope (TEM/HRTEM) and UV-vis spectroscopy. By TEM we observed that spherical Au NPs were successfully prepared with average diameter of 1.5-5.9 nm. HRTEM revealed that a majority of them is rather of a round or irregular shape with a frequent multiple twinning. Dynamic light scattering as well as TEM confirmed that Au NPs have monodal size distribution. According to atomic absorption spectroscopy, concentration of gold increases with sputtering time and does not change with aging time. PEG-SH exhibited loss of SPR absorption band in UV-vis spectrum because of the small NP size (1.5 nm). PEG and PEG-NH2 showed good thermal and aging stability. No significant antibacterial effect of any type of the studied Au NPs has been discovered. Cytotoxic effect on osteoblasts was determined only with the highest concentration of Au NP with PEG and PEG-NH2 stabilization. Au NPs stabilized by PEG-SH showed the overall optimal physical and biological properties for drug delivery applications.
Název v anglickém jazyce
PEGylated gold nanoparticles: Stability, cytotoxicity and antibacterial activity
Popis výsledku anglicky
Designing safe nanomaterials is nowadays a critical issue. A way to prepare PEGylated Au NPs by sputtering of Au into pure (PEG), thiol (PEG-SH) and amine terminated polyethylene glycols (PEG-NH2) was explored. Structural characterizations, aging and thermal stability were performed using transmission electron microscope (TEM/HRTEM) and UV-vis spectroscopy. By TEM we observed that spherical Au NPs were successfully prepared with average diameter of 1.5-5.9 nm. HRTEM revealed that a majority of them is rather of a round or irregular shape with a frequent multiple twinning. Dynamic light scattering as well as TEM confirmed that Au NPs have monodal size distribution. According to atomic absorption spectroscopy, concentration of gold increases with sputtering time and does not change with aging time. PEG-SH exhibited loss of SPR absorption band in UV-vis spectrum because of the small NP size (1.5 nm). PEG and PEG-NH2 showed good thermal and aging stability. No significant antibacterial effect of any type of the studied Au NPs has been discovered. Cytotoxic effect on osteoblasts was determined only with the highest concentration of Au NP with PEG and PEG-NH2 stabilization. Au NPs stabilized by PEG-SH showed the overall optimal physical and biological properties for drug delivery applications.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
20501 - Materials engineering
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Colloids and Surfaces A: Physicochemical and Engineering Aspects
ISSN
0927-7757
e-ISSN
—
Svazek periodika
560
Číslo periodika v rámci svazku
JAN 5 2019
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
26-34
Kód UT WoS článku
000451048400004
EID výsledku v databázi Scopus
—