Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918743" target="_blank" >RIV/60461373:22310/19:43918743 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/19:43918743 RIV/44555601:13440/19:43894943

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1381514819304833?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1381514819304833?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.reactfunctpolym.2019.104339" target="_blank" >10.1016/j.reactfunctpolym.2019.104339</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation

Popis výsledku v původním jazyce

In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70% ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70% ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition Of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70% ethanol was preferable for SH-SY5Y attachment, growth and migration.

Název v anglickém jazyce

Effect of sterilization methods on electrospun cellulose acetate butyrate nanofibers for SH-SY5Y cultivation

Popis výsledku anglicky

In the biomedical field, the sterilization of nanofibers is of particular importance because it can significantly affect their morphology, physicochemical properties and cytocompatibility. Here, we studied the effect of conventional sterilization with 70% ethanol or ultraviolet (UV) radiation on cellulose acetate butyrate (CAB) nanofibers. Scanning electron microscopy (SEM) revealed that short-term (30 min) UV sterilization had no influence on nanofiber morphology while 70% ethanol led to swelling and, thus, reduced porosity. Water contact angle (WCA) measurements confirmed an increase in wettability after sterilization with ethanol. Fourier transform spectroscopy (FTIR) and electrokinetic analyses indicated only slight changes in the chemical composition Of the CAB nanofibers for both sterilization types. However, longer sterilization time (2 h) led to notable changes in the chemical structure of the scaffolds. In turn, X-ray photoelectron spectroscopy (XPS) revealed changes in the chemical composition, even after short-term sterilization. In combination with FTIR and XPS, differential scanning calorimetry (DSC) identified the hydrolysis of the ethanol-sterilized scaffold and degradation of UV-sterilized one. Human neuroblastoma cell (SH-SY5Y) cultivation on the CAB scaffolds demonstrated significant changes in cell morphology and viability depending on sterilization type. Sterilization of CAB with 70% ethanol was preferable for SH-SY5Y attachment, growth and migration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/GA17-00885S" target="_blank" >GA17-00885S: Nanostrukturované nosiče na bázi celulózy a jejích derivátů s nastavitelnými vlastnostmi pro tkáňové inženýrství</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reactive and Functional Polymers

ISSN

1381-5148

e-ISSN

—

Svazek periodika

143

Číslo periodika v rámci svazku

OCT 2019

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

8

Strana od-do

"UNSP 104339/1"-8

Kód UT WoS článku

000489195400003

EID výsledku v databázi Scopus

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