N-(2-Hydroxyethyl)-L-valyl-L-leucine: a novel urinary biomarker of ethylene oxide exposure in humans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F20%3A43920720" target="_blank" >RIV/60461373:22310/20:43920720 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/75010330:_____/20:00012998

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S037842742030076X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S037842742030076X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxlet.2020.03.004" target="_blank" >10.1016/j.toxlet.2020.03.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

N-(2-Hydroxyethyl)-L-valyl-L-leucine: a novel urinary biomarker of ethylene oxide exposure in humans

Popis výsledku v původním jazyce

Ethylene oxide (EO), a carcinogenic chemical used as an industrial intermediate and sterilant, forms covalent adducts with DNA and proteins. The adduct with N-terminal valine [N-(2-hydroxyethyl)-L-valine, HEV] in blood protein globin has been employed as a principal biomarker of cumulative exposures to EO. However, as sampling of blood is inconvenient in routine occupational health practice, a non-invasive alternative to globin analysis has been investigated. Following identification of N-(2-hydroxyethyl)-L-valyl-L-leucine (HEVL) as ultimate cleavage product of EO-adducted globin excreted in the rat urine, here we report for the first time on the presence of HEVL in the urine of humans. In 18 sterilization workers, urinary HEVL ranged from 0.67 to 11.98 μg/g creatinine (mean ± SD: 5.04 ± 3.14 μg/g creat) and correlated with HEV: HEVL (μg/g creat) = 0.833 HEV (nmol/g globin) + 1.19 (R2 = 0.45). As unexpectedly high levels of urinary HEVL were found also in controls (mean ± SD: 0.97 ± 0.37 μg/g creat, n = 32), HEVL is not proposed for the accurate assessment of sub-ppm exposures to EO. On the other hand, non-invasive sampling and facile work-up procedure predetermine HEVL for screening purposes to identify subjects approaching to or exceeding occupational exposure limit for EO (1.8 mg/m3) to be re-examined by the more sensitive reference analysis for HEV. © 2020 Elsevier B.V.

Název v anglickém jazyce

N-(2-Hydroxyethyl)-L-valyl-L-leucine: a novel urinary biomarker of ethylene oxide exposure in humans

Popis výsledku anglicky

Ethylene oxide (EO), a carcinogenic chemical used as an industrial intermediate and sterilant, forms covalent adducts with DNA and proteins. The adduct with N-terminal valine [N-(2-hydroxyethyl)-L-valine, HEV] in blood protein globin has been employed as a principal biomarker of cumulative exposures to EO. However, as sampling of blood is inconvenient in routine occupational health practice, a non-invasive alternative to globin analysis has been investigated. Following identification of N-(2-hydroxyethyl)-L-valyl-L-leucine (HEVL) as ultimate cleavage product of EO-adducted globin excreted in the rat urine, here we report for the first time on the presence of HEVL in the urine of humans. In 18 sterilization workers, urinary HEVL ranged from 0.67 to 11.98 μg/g creatinine (mean ± SD: 5.04 ± 3.14 μg/g creat) and correlated with HEV: HEVL (μg/g creat) = 0.833 HEV (nmol/g globin) + 1.19 (R2 = 0.45). As unexpectedly high levels of urinary HEVL were found also in controls (mean ± SD: 0.97 ± 0.37 μg/g creat, n = 32), HEVL is not proposed for the accurate assessment of sub-ppm exposures to EO. On the other hand, non-invasive sampling and facile work-up procedure predetermine HEVL for screening purposes to identify subjects approaching to or exceeding occupational exposure limit for EO (1.8 mg/m3) to be re-examined by the more sensitive reference analysis for HEV. © 2020 Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30305 - Occupational health

Projekt

<a href="/cs/project/NV19-09-00378" target="_blank" >NV19-09-00378: Štěpné produkty proteinových aduktů v moči jako nový typ biomarkerů v preventivní medicíně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

—

Svazek periodika

326

Číslo periodika v rámci svazku

bez čísla

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

18-22

Kód UT WoS článku

000522851200003

EID výsledku v databázi Scopus

2-s2.0-85080973338