The effect of chemical structure of OEG ligand shells with quaternary ammonium moiety on the colloidal stabilization, cellular uptake and photothermal stability of gold nanorods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F21%3A43933915" target="_blank" >RIV/60461373:22310/21:43933915 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/21:00544878 RIV/00179906:_____/21:10428287 RIV/68407700:21340/21:00350319 RIV/62690094:18470/21:50018089

Výsledek na webu

<a href="https://www.dovepress.com/the-effect-of-chemical-structure-of-oeg-ligand-shells-with-quaternary--peer-reviewed-fulltext-article-IJN" target="_blank" >https://www.dovepress.com/the-effect-of-chemical-structure-of-oeg-ligand-shells-with-quaternary--peer-reviewed-fulltext-article-IJN</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2147/IJN.S304953" target="_blank" >10.2147/IJN.S304953</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of chemical structure of OEG ligand shells with quaternary ammonium moiety on the colloidal stabilization, cellular uptake and photothermal stability of gold nanorods

Popis výsledku v původním jazyce

Purpose: Plasmonic photothermal cancer therapy by gold nanorods (GNRs) emerges as a promising tool for cancer treatment. The goal of this study was to design cationic oligoethylene glycol (OEG) compounds varying in hydrophobicity and molecular electrostatic potential as ligand shells of GNRs. Three series of ligands with different length of OEG chain (ethylene glycol units = 3, 4, 5) and variants of quaternary ammonium salts (QAS) as terminal functional group were synthesized and compared to a prototypical quaternary ammonium ligand with alkyl chain - (16-mercaptohexadecyl)trimethylammonium bromide (MTAB). Methods: Step-by-step research approach starting with the preparation of compounds characterized by NMR and HRMS spectra, GNRs ligand exchange evaluation through characterization of cytotoxicity and GNRs cellular uptake was used. A method quantifying the reshaping of GNRs was applied to determine the effect of ligand structure on the heat transport from GNRs under fs-laser irradiation. Results: Fourteen out of 18 synthesized OEG compounds successfully stabilized GNRs in the water. The colloidal stability of prepared GNRs in the cell culture medium decreased with the number of OEG units. In contrast, the cellular uptake of OEG+GNRs by HeLa cells increased with the length of OEG chain while the structure of the QAS group showed a minor role. Compared to MTAB, more hydrophilic OEG compounds exhibited nearly two order of magnitude lower cytotoxicity in free state and provided efficient cellular uptake of GNRs close to the level of MTAB. Regarding photothermal properties, OEG compounds evoked the photothermal reshaping of GNRs at lower peak fluence (14.8 mJ/cm2) of femtosecond laser irradiation than the alkanethiol MTAB. Conclusion: GNRs appear to be optimal for clinical applications with systemic administration of NPs not-requiring irradiation at high laser intensity such as drug delivery and photothermal therapy inducing apoptosis. © 2021 Salajkova et al.

Název v anglickém jazyce

The effect of chemical structure of OEG ligand shells with quaternary ammonium moiety on the colloidal stabilization, cellular uptake and photothermal stability of gold nanorods

Popis výsledku anglicky

Purpose: Plasmonic photothermal cancer therapy by gold nanorods (GNRs) emerges as a promising tool for cancer treatment. The goal of this study was to design cationic oligoethylene glycol (OEG) compounds varying in hydrophobicity and molecular electrostatic potential as ligand shells of GNRs. Three series of ligands with different length of OEG chain (ethylene glycol units = 3, 4, 5) and variants of quaternary ammonium salts (QAS) as terminal functional group were synthesized and compared to a prototypical quaternary ammonium ligand with alkyl chain - (16-mercaptohexadecyl)trimethylammonium bromide (MTAB). Methods: Step-by-step research approach starting with the preparation of compounds characterized by NMR and HRMS spectra, GNRs ligand exchange evaluation through characterization of cytotoxicity and GNRs cellular uptake was used. A method quantifying the reshaping of GNRs was applied to determine the effect of ligand structure on the heat transport from GNRs under fs-laser irradiation. Results: Fourteen out of 18 synthesized OEG compounds successfully stabilized GNRs in the water. The colloidal stability of prepared GNRs in the cell culture medium decreased with the number of OEG units. In contrast, the cellular uptake of OEG+GNRs by HeLa cells increased with the length of OEG chain while the structure of the QAS group showed a minor role. Compared to MTAB, more hydrophilic OEG compounds exhibited nearly two order of magnitude lower cytotoxicity in free state and provided efficient cellular uptake of GNRs close to the level of MTAB. Regarding photothermal properties, OEG compounds evoked the photothermal reshaping of GNRs at lower peak fluence (14.8 mJ/cm2) of femtosecond laser irradiation than the alkanethiol MTAB. Conclusion: GNRs appear to be optimal for clinical applications with systemic administration of NPs not-requiring irradiation at high laser intensity such as drug delivery and photothermal therapy inducing apoptosis. © 2021 Salajkova et al.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21000 - Nano-technology

Projekt

<a href="/cs/project/EF15_003%2F0000444" target="_blank" >EF15_003/0000444: Pokročilí funkční nanoroboti</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Nanomedicine

ISSN

1178-2013

e-ISSN

1178-2013

Svazek periodika

16

Číslo periodika v rámci svazku

MAY 2021

Stát vydavatele periodika

NZ - Nový Zéland

Počet stran výsledku

21

Strana od-do

3407-3427

Kód UT WoS článku

000651874100001

EID výsledku v databázi Scopus

2-s2.0-85106927892