Development of Smart Sensing Film with Nonbiofouling Properties for Potentiometric Detection of Local pH Changes Caused by Bacterial and Yeast Infections Around Orthopedic Implants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F22%3A43925151" target="_blank" >RIV/60461373:22310/22:43925151 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/23:00568558 RIV/68407700:21220/23:00361293 RIV/00216208:11110/23:10451750 RIV/00064203:_____/23:10451750

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/10.1002/admi.202201878" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/admi.202201878</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/admi.202201878" target="_blank" >10.1002/admi.202201878</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of Smart Sensing Film with Nonbiofouling Properties for Potentiometric Detection of Local pH Changes Caused by Bacterial and Yeast Infections Around Orthopedic Implants

Popis výsledku v původním jazyce

The local peri-implant pH changes caused by sterile inflammation and bacterial and fungal infections are studied herein. Then, a sensing electrode based on polyaniline and poly(2-methyl-2-oxazoline) on a titanium alloy support is developed for potentiometric detection of peri-implant pH changes to enable early detection of the aforementioned pathologies. The infected endoprosthesis area is shown to have an average pH of 0.79 units lower than the aseptic sample. The pH measurements of the individual pathogenic bacteria or pathogenic yeast reveal that Escherichia coli decreased the pH by 1.24 units, Staphylococcus aureus decreased the pH by 1.33 units and the methicillin-resistant Staphylococcus aureus bacteria decreased the pH from 7.2 to 5.6 during 10 h, followed by a subsequent increase to 6.4. The results are statistically significant (α = 0.01). Pseudomonas aeruginosa is not shown to change pH levels. On the other hand, the pathogenic yeast has the lowest recorded pH, which decreases from 5.8 to 4.8. This difference in pH can be used to identify the nature of the infection. The developed electrodes have a pH response between pH 5 and 8, with a Nernstian slope of −59.6/pH. The developed electrode can contribute to the next generation of biosensors

Název v anglickém jazyce

Development of Smart Sensing Film with Nonbiofouling Properties for Potentiometric Detection of Local pH Changes Caused by Bacterial and Yeast Infections Around Orthopedic Implants

Popis výsledku anglicky

The local peri-implant pH changes caused by sterile inflammation and bacterial and fungal infections are studied herein. Then, a sensing electrode based on polyaniline and poly(2-methyl-2-oxazoline) on a titanium alloy support is developed for potentiometric detection of peri-implant pH changes to enable early detection of the aforementioned pathologies. The infected endoprosthesis area is shown to have an average pH of 0.79 units lower than the aseptic sample. The pH measurements of the individual pathogenic bacteria or pathogenic yeast reveal that Escherichia coli decreased the pH by 1.24 units, Staphylococcus aureus decreased the pH by 1.33 units and the methicillin-resistant Staphylococcus aureus bacteria decreased the pH from 7.2 to 5.6 during 10 h, followed by a subsequent increase to 6.4. The results are statistically significant (α = 0.01). Pseudomonas aeruginosa is not shown to change pH levels. On the other hand, the pathogenic yeast has the lowest recorded pH, which decreases from 5.8 to 4.8. This difference in pH can be used to identify the nature of the infection. The developed electrodes have a pH response between pH 5 and 8, with a Nernstian slope of −59.6/pH. The developed electrode can contribute to the next generation of biosensors

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Materials Interfaces

ISSN

2196-7350

e-ISSN

—

Svazek periodika

2201878

Číslo periodika v rámci svazku

2201878

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000895528500001

EID výsledku v databázi Scopus

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