Activation of Lipase from Geotrichum candidum and its Enantioselectivity towards Xenobiotics Substrates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008941" target="_blank" >RIV/60461373:22330/03:00008941 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/03:00017974

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Activation of Lipase from Geotrichum candidum and its Enantioselectivity towards Xenobiotics Substrates

Popis výsledku v původním jazyce

Convenient procedures were developed for stimulation of lipase activity with specificity towards natural polyunsaturated fatty acids. Four strains of Geotrichum candidum were tested during this study. Lipase activity was determined and proved in the usedstrains, and for majority of tested media. Olive oil was employed as activator for the determination of the lipase activity. Calculated differences in lipase activity values were used as basis for optimizing of fermentation conditions. Majority of activated lipases represented extracellular lipases, which penetrated through cellular membrane into the medium containing activator. The activity of intracellular lipases was determined as well. To evaluate different media, that one containing peptone as nitrogen source was proved to be the best one of the tested series of media. The most remarkable growth of the cell biomass was also observed using this medium. The highest specific activity of all activated lipases was found in the second h

Název v anglickém jazyce

Activation of Lipase from Geotrichum candidum and its Enantioselectivity towards Xenobiotics Substrates

Popis výsledku anglicky

Convenient procedures were developed for stimulation of lipase activity with specificity towards natural polyunsaturated fatty acids. Four strains of Geotrichum candidum were tested during this study. Lipase activity was determined and proved in the usedstrains, and for majority of tested media. Olive oil was employed as activator for the determination of the lipase activity. Calculated differences in lipase activity values were used as basis for optimizing of fermentation conditions. Majority of activated lipases represented extracellular lipases, which penetrated through cellular membrane into the medium containing activator. The activity of intracellular lipases was determined as well. To evaluate different media, that one containing peptone as nitrogen source was proved to be the best one of the tested series of media. The most remarkable growth of the cell biomass was also observed using this medium. The highest specific activity of all activated lipases was found in the second h

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F02%2F0166" target="_blank" >GA203/02/0166: Glykosidy a glykuronáty biologicky aktivních alkoholů jako potenciální prostředky při ochraně před hmyzími škůdci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemické listy

ISSN

0009-2770

e-ISSN

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Svazek periodika

97

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

2

Strana od-do

293-294

Kód UT WoS článku

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EID výsledku v databázi Scopus

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