In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008957" target="_blank" >RIV/60461373:22330/03:00008957 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus

Popis výsledku v původním jazyce

Two types of phospholipid degrading enzymes phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl inositol specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two typesof activities; polyphosphoinositide requiring (PIP2-dependent PLD) and polyphosphoinositide independent requiring millimolar concentration of calcium (PLD?). Significantly different patterns of the activity profiles were found for soluble and membrane associated forms of all three enzymes within both processes. Membrane associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD?, while highest PI-PLC activity appears in the same stages of development of seeds and plants as forPLD?. In subcellular fractions of hypocotyls parts of young plants phospholipases were localized predominantly on plasma membranes. Biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles isola

Název v anglickém jazyce

In vitro distribution and characterisation of membrane associated PLD and PI-PLC in Brassica napus

Popis výsledku anglicky

Two types of phospholipid degrading enzymes phospholipase D (PLD; EC 3.1.4.4) and phosphatidyl inositol specific phospholipase C (PIP2-PLC; PI-PLC 3.1.4.11) were studied during the development of seeds and plants of Brassica napus. PLD exhibits two typesof activities; polyphosphoinositide requiring (PIP2-dependent PLD) and polyphosphoinositide independent requiring millimolar concentration of calcium (PLD?). Significantly different patterns of the activity profiles were found for soluble and membrane associated forms of all three enzymes within both processes. Membrane associated PIP2-dependent PLD activity shows the opposite trend when compared to PLD?, while highest PI-PLC activity appears in the same stages of development of seeds and plants as forPLD?. In subcellular fractions of hypocotyls parts of young plants phospholipases were localized predominantly on plasma membranes. Biochemical characteristics (Ca2+, pH) of all three enzymes associated with plasma membrane vesicles isola

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F00%2F1332" target="_blank" >GA522/00/1332: Úloha fosfolipas v mechanismu přenosu signálů u rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

2003

Číslo periodika v rámci svazku

54

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

691-698

Kód UT WoS článku

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EID výsledku v databázi Scopus

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