Identification of isoflavonoids in Arabidopsis thaliana.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012974" target="_blank" >RIV/60461373:22330/04:00012974 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

čeština

Název v původním jazyce

Identification of isoflavonoids in Arabidopsis thaliana.

Popis výsledku v původním jazyce

Extracts from leaves and inflorescence stalks of Arabidopsis thaliana and from seedlings of Lepidium sativa (Brassicaceae) were analyzed for the presence of isoflavonoids by HPLC-MS-SIM and by five isoflavonoid-specific ELISA methods after the HPLC fractionation of samples (1). Both analytical approaches revealed homologous spectra of isoflavonoids in both plant species. Formononetin and prunetin represented the main aglycones followed by biochanin A, daidzein and genistein; sissotrin was the most abundant isoflavonoid glycoside followed by ononin, daidzin and genistin. The content of individual compounds ranged from a few micrograms up to 2.2 milligrams per kilogram (dry weight). Genes homologous to enzymes involved in isoflavonoid synthesis and metabolism found in the Arabidopsis thaliana genome and their expression profiles were extracted from number of transcriptomic datasets. Genes likely to be involved in important steps of the isoflavonoid metabolism in Arabidopsis thaliana were

Název v anglickém jazyce

Identification of isoflavonoids in Arabidopsis thaliana.

Popis výsledku anglicky

Extracts from leaves and inflorescence stalks of Arabidopsis thaliana and from seedlings of Lepidium sativa (Brassicaceae) were analyzed for the presence of isoflavonoids by HPLC-MS-SIM and by five isoflavonoid-specific ELISA methods after the HPLC fractionation of samples (1). Both analytical approaches revealed homologous spectra of isoflavonoids in both plant species. Formononetin and prunetin represented the main aglycones followed by biochanin A, daidzein and genistein; sissotrin was the most abundant isoflavonoid glycoside followed by ononin, daidzin and genistin. The content of individual compounds ranged from a few micrograms up to 2.2 milligrams per kilogram (dry weight). Genes homologous to enzymes involved in isoflavonoid synthesis and metabolism found in the Arabidopsis thaliana genome and their expression profiles were extracted from number of transcriptomic datasets. Genes likely to be involved in important steps of the isoflavonoid metabolism in Arabidopsis thaliana were

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F03%2F0352" target="_blank" >GA525/03/0352: Isoflavonoidy v čeledích Myrtaceae a Rutaceae</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Chemické listy

ISBN

0009-2770

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

1022-1023

Název nakladatele

Česká chemická společnost

Místo vydání

Praha

Místo konání akce

Nymburk

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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