Metody identifikace rhisosférních mikroorganosmů degradující PCB

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012984" target="_blank" >RIV/60461373:22330/04:00012984 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/04:00019590

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods for identification of rhizosperic bacteria degrading PCB and Approach to identify them by molecular biology method

Popis výsledku v původním jazyce

This work is based on research which focuses on the study of the possible mutual relationship of microorganisms and plants in PCB metabolism. Three different plant species - Nicotiana tabacum (tobacco), Solanum nigrum (black nightshade) and Medicago sativa (alfalfa) - were cultivated in real soil from an industrial site, which is long-term contaminated by PCBs. After six months the decrease of PCB content in soil and the PCB accumulation in plant tissue was analysed. The highest decrease of PCB concentration was measured from the soil vegetated with tobacco (34% decrease compared to bulk soil). From this polluted soil, bacteria which potentially participate in the biodegradation of PCBs were isolated and were screened for the presence of bphA1 and bphCgenes. These genes are apart of the bph operon, which is very important for PCB degradation. The PCR primers were designed for conserved regions of bphA1 and bphC genes from an alignment of previously published sequences. The degradative

Název v anglickém jazyce

Methods for identification of rhizosperic bacteria degrading PCB and Approach to identify them by molecular biology method

Popis výsledku anglicky

This work is based on research which focuses on the study of the possible mutual relationship of microorganisms and plants in PCB metabolism. Three different plant species - Nicotiana tabacum (tobacco), Solanum nigrum (black nightshade) and Medicago sativa (alfalfa) - were cultivated in real soil from an industrial site, which is long-term contaminated by PCBs. After six months the decrease of PCB content in soil and the PCB accumulation in plant tissue was analysed. The highest decrease of PCB concentration was measured from the soil vegetated with tobacco (34% decrease compared to bulk soil). From this polluted soil, bacteria which potentially participate in the biodegradation of PCBs were isolated and were screened for the presence of bphA1 and bphCgenes. These genes are apart of the bph operon, which is very important for PCB degradation. The PCR primers were designed for conserved regions of bphA1 and bphC genes from an alignment of previously published sequences. The degradative

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/GA526%2F01%2F1292" target="_blank" >GA526/01/1292: Studium metabolismu a vztahu rhizosferních mikroorganismů s rostlinnými systémy při odbourávání organických sloučenin kontaminujících životní prostředí</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Conference Rhizoremediace

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

254

Název nakladatele

Hartmann A.

Místo vydání

Mnichov

Místo konání akce

Mnichpv

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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