Metody pro studium isoflavonoidů v non-leguminózních rostlinách.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F04%3A00012985" target="_blank" >RIV/60461373:22330/04:00012985 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methods for study of isoflavonoids in non-leguminous plants

Popis výsledku v původním jazyce

Isoflavonoids (3-phenylchromones) are most abundant in the Fabaceae, however they were recorded in over 20 other families, dicotyledons (e.g. Rosaceae, Asteraceae) as well as monocots (e.g. Iridaceae, Poaceae). Molecular biology data indicate occurrenceof genes for isoflavonoid metabolic pathway even in higher number of families, nevertheless it is difficult to obtain the evidence of their biochemical activity. In our recent work, we combine HPLC-MS and immunochemical approaches. A set of five immunoassays for early products of isoflavonoid metabolic pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) was adapted for phytochemical studies. Due to the narrow specificities and high sensitivities of these assays (detection limit below 10 pg), the initial screening may be performed in a large number of simply prepared samples, e.g. in diluted water-ethanolic extracts. Immunoanalysis of crude extracts yields semi-quantitative data, enabling to optimize the

Název v anglickém jazyce

Methods for study of isoflavonoids in non-leguminous plants

Popis výsledku anglicky

Isoflavonoids (3-phenylchromones) are most abundant in the Fabaceae, however they were recorded in over 20 other families, dicotyledons (e.g. Rosaceae, Asteraceae) as well as monocots (e.g. Iridaceae, Poaceae). Molecular biology data indicate occurrenceof genes for isoflavonoid metabolic pathway even in higher number of families, nevertheless it is difficult to obtain the evidence of their biochemical activity. In our recent work, we combine HPLC-MS and immunochemical approaches. A set of five immunoassays for early products of isoflavonoid metabolic pathway (i.e. daidzein, formononetin, genistein and biochanin A and their glycosides) was adapted for phytochemical studies. Due to the narrow specificities and high sensitivities of these assays (detection limit below 10 pg), the initial screening may be performed in a large number of simply prepared samples, e.g. in diluted water-ethanolic extracts. Immunoanalysis of crude extracts yields semi-quantitative data, enabling to optimize the

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F03%2F0352" target="_blank" >GA525/03/0352: Isoflavonoidy v čeledích Myrtaceae a Rutaceae</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Polyphenols Communications

ISBN

952-10-1977-8

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

623-624

Název nakladatele

neuveden

Místo vydání

Helsinky

Místo konání akce

Helsinky

Datum konání akce

1. 1. 2004

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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