Studie in vitro skládání CA-NC kapsid Mason-Pfizerova opičího viru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00015563" target="_blank" >RIV/60461373:22330/05:00015563 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/05:00016398

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids

Popis výsledku v původním jazyce

In contrast to other retroviruses as HIV, Mason-Pfizer monkey virus (M-PMV) preassembles immature capsids in the cytoplasm. We have performed the in vitro assembly of purified bacterially expressed proteins to address the difference of morphogenically distinct retroviruses M-PMV and HIV in their ability to assemble VLPs from minimized assembly competent domains. The fusion capsid and nucleocapsid protein (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of mature andimmature particles, respectively. We found dramatically different ability to assemble CANC particles between these two viruses. Also ProCANC particles of M-PMV differed from that of HIV forming spheres and tubes, respectively. The requirements for factors affecting assembly were also evaluated and the amount of incorporated fluorescently labeled nucleic acids was determined. The presence of purified RNA efficiently promoted formation of ProCANC spherical particles. The oligonucleotides

Název v anglickém jazyce

In vitro study of assembly of Mason-Pfizer monkey virus CA-NC capsids

Popis výsledku anglicky

In contrast to other retroviruses as HIV, Mason-Pfizer monkey virus (M-PMV) preassembles immature capsids in the cytoplasm. We have performed the in vitro assembly of purified bacterially expressed proteins to address the difference of morphogenically distinct retroviruses M-PMV and HIV in their ability to assemble VLPs from minimized assembly competent domains. The fusion capsid and nucleocapsid protein (CANC) and its N-terminally modified mutant ( ProCANC) were used to mimic the assembly of mature andimmature particles, respectively. We found dramatically different ability to assemble CANC particles between these two viruses. Also ProCANC particles of M-PMV differed from that of HIV forming spheres and tubes, respectively. The requirements for factors affecting assembly were also evaluated and the amount of incorporated fluorescently labeled nucleic acids was determined. The presence of purified RNA efficiently promoted formation of ProCANC spherical particles. The oligonucleotides

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů