New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017745" target="_blank" >RIV/60461373:22330/06:00017745 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin

Popis výsledku v původním jazyce

The new method exploits the characteristic of staphylococcal protease V8, which specifically splits peptide bonds where l-glutamic or l-aspartic acid participate. Those peptide bonds where d-aspartic acid is present remain unsplit because of the stereospecifity of enzymes. In accordance with expectations, fewer peptide bonds are split by this protease at more advanced ages and larger peptide fragments are thus formed due to the higher content of d-amino acid residues in the proteins of older people. Thesamples of acid-extracted non-collagenous proteins from dentin were separated using high performance liquid chromatography after enzymatic hydrolysis. A peak with a retention time of 45.3 min was chosen and his enlarging area showed a linear correlationwith increasing age. Although the linear correlation with age was proved, the scattering of values decreases the usefulness of the proposed method for age estimation.

Název v anglickém jazyce

New Method of Age Estimation based on the Changes in Human Non-collagenous Proteins from Dentin

Popis výsledku anglicky

The new method exploits the characteristic of staphylococcal protease V8, which specifically splits peptide bonds where l-glutamic or l-aspartic acid participate. Those peptide bonds where d-aspartic acid is present remain unsplit because of the stereospecifity of enzymes. In accordance with expectations, fewer peptide bonds are split by this protease at more advanced ages and larger peptide fragments are thus formed due to the higher content of d-amino acid residues in the proteins of older people. Thesamples of acid-extracted non-collagenous proteins from dentin were separated using high performance liquid chromatography after enzymatic hydrolysis. A peak with a retention time of 45.3 min was chosen and his enlarging area showed a linear correlationwith increasing age. Although the linear correlation with age was proved, the scattering of values decreases the usefulness of the proposed method for age estimation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Forensic Science International

ISSN

0379-0738

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

156

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

245-249

Kód UT WoS článku

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EID výsledku v databázi Scopus

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