Molekulární charakterizace termofilních a mezofilních druhů rodu Bacillus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F06%3A00017798" target="_blank" >RIV/60461373:22330/06:00017798 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of thermophilic and mesophilic Bacillus species

Popis výsledku v původním jazyce

Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. All above mentioned techniques have been optimized to be able to reach special electrophoresis pattern and discriminate among different species. RAPD protocol was optimized by using two different primers which both have been found to be effective for the RAPD fingerprinting of assessed bacilli. Amplification of the 16S-23S rRNA ITS produced multiple bands in gel electrophoresis, implying multiple copies of variable length in the ITS of these bacteria. The ITS groupings were easier to interpret than the RAPD types and hence ITS ty

Název v anglickém jazyce

Molecular characterization of thermophilic and mesophilic Bacillus species

Popis výsledku anglicky

Molecular biology assays were used to determine the identification of thermophilic and mesophilic bacilli. A genotypic study using randomly amplified polymorphic DNA analysis (RAPD), amplification of the 16S-23S rRNA intergenic transcribed spacer (ITS-PCR), restriction fragment length polymorphism (RFLP) of 16S rRNA and restriction fragment length polymorphism of the ITS-PCR (ITS-PCR RFLP) was carried out with 5 thermophilic and 3 mesophilic Bacillus isolates. All above mentioned techniques have been optimized to be able to reach special electrophoresis pattern and discriminate among different species. RAPD protocol was optimized by using two different primers which both have been found to be effective for the RAPD fingerprinting of assessed bacilli. Amplification of the 16S-23S rRNA ITS produced multiple bands in gel electrophoresis, implying multiple copies of variable length in the ITS of these bacteria. The ITS groupings were easier to interpret than the RAPD types and hence ITS ty

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

WSEAS Transactions on Biology and Biomedicine

ISSN

1109-9518

e-ISSN

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Svazek periodika

3

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

4

Strana od-do

27-30

Kód UT WoS článku

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EID výsledku v databázi Scopus

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