Radioimmunoassay of 16?-Hydroxy-Dehydroepiandrosteronu a jeho fyziologické hladiny

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F07%3A00018983" target="_blank" >RIV/60461373:22330/07:00018983 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Radioimmunoassay of 16?-Hydroxy-Dehydroepiandrosterone and its Physiological levels

Popis výsledku v původním jazyce

16?-Hydroxy-dehydroepiandrosterone (16?-OH-DHEA) belongs to the products of extensive DHEA metabolism in mammalian tissues. It is a precursor of 16?-hydroxylated estrogens, increased levels of which are associated with autoimmune disorders. A highly specific radioimmunoassay of unconjugated 16?-OH-DHEA was developed and evaluated. Polyclonal rabbit antisera were raised against 3?,16?- dihydroxy-17,19-dione-19-O-(carboxymethyloxime) and 3?,16?-dihydroxy-7,17-dione-7-O-(carboxymethyloxime) BSA conjugates.Two methods were used for preparation of the conjugates. Homologous radioiodinated derivatives with tyrosine methyl ester were prepared as tracers. While antisera to 7-CMO cross-reacted with DHEA as much as by 58%, the cross-reaction of the chosen antiserum prepared via 19-oxogroup by micellar conjugation technique with 16?-OH-DHEA was only 0.13% and with all other structurally related steroids, including DHEA were lower than 0.01%. The detection limi

Název v anglickém jazyce

Radioimmunoassay of 16?-Hydroxy-Dehydroepiandrosterone and its Physiological levels

Popis výsledku anglicky

16?-Hydroxy-dehydroepiandrosterone (16?-OH-DHEA) belongs to the products of extensive DHEA metabolism in mammalian tissues. It is a precursor of 16?-hydroxylated estrogens, increased levels of which are associated with autoimmune disorders. A highly specific radioimmunoassay of unconjugated 16?-OH-DHEA was developed and evaluated. Polyclonal rabbit antisera were raised against 3?,16?- dihydroxy-17,19-dione-19-O-(carboxymethyloxime) and 3?,16?-dihydroxy-7,17-dione-7-O-(carboxymethyloxime) BSA conjugates.Two methods were used for preparation of the conjugates. Homologous radioiodinated derivatives with tyrosine methyl ester were prepared as tracers. While antisera to 7-CMO cross-reacted with DHEA as much as by 58%, the cross-reaction of the chosen antiserum prepared via 19-oxogroup by micellar conjugation technique with 16?-OH-DHEA was only 0.13% and with all other structurally related steroids, including DHEA were lower than 0.01%. The detection limi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Steroid Biochemistry and Molecular Biology

ISSN

0960-0760

e-ISSN

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Svazek periodika

104

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

130-135

Kód UT WoS článku

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EID výsledku v databázi Scopus

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