Vyhodnocení poškození DNA vyvolané reakcí kyslíku a inhibiční efekt hemových bílkoviny na toto poškození

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021070" target="_blank" >RIV/60461373:22330/08:00021070 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage

Popis výsledku v původním jazyce

Reactive oxygen species (ROS), e.g., hydrogen peroxide, are aerobic cellular by products, where excess oxidatively damages DNA, proteins and lipids. This is of particular concern regarding carcinogenesis. The Fenton reaction generates ROS. Several reports suggest haemoglobin-containing heme-iron, like other iron-rich substances, produce ROS in vitro due to this catalyst. As haemoglobin has been considered a villain, playing a negative role for human health in vitro, this potentially damaging effect on cellular DNA requires clarification in vivo. Here, we show that under the coexistence of haemoglobin derivatives and heme with hydrogen peroxide, DNA did not damage. This inhibition was confirmed in cell culture using a single cell gel electrophoresis assay, comet assay, proving haemoglobin has catalase activity. These results dispute prior findings that in vitro haemoglobin generates ROS, which damages DNA-inducing carcinogenesis. In fact, haemoglobin delays or inhibits carcinogenesis ca

Název v anglickém jazyce

Evaluation of DNA damage induced with reactive oxygen and inhibitory effect of hem protein on its damage

Popis výsledku anglicky

Reactive oxygen species (ROS), e.g., hydrogen peroxide, are aerobic cellular by products, where excess oxidatively damages DNA, proteins and lipids. This is of particular concern regarding carcinogenesis. The Fenton reaction generates ROS. Several reports suggest haemoglobin-containing heme-iron, like other iron-rich substances, produce ROS in vitro due to this catalyst. As haemoglobin has been considered a villain, playing a negative role for human health in vitro, this potentially damaging effect on cellular DNA requires clarification in vivo. Here, we show that under the coexistence of haemoglobin derivatives and heme with hydrogen peroxide, DNA did not damage. This inhibition was confirmed in cell culture using a single cell gel electrophoresis assay, comet assay, proving haemoglobin has catalase activity. These results dispute prior findings that in vitro haemoglobin generates ROS, which damages DNA-inducing carcinogenesis. In fact, haemoglobin delays or inhibits carcinogenesis ca

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fleischwirtschaft International

ISSN

0179-2415

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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