Produkce rekombinantních proteinů buňkami Picha pastoris Mut+ rostoucími v kontinuální kultuře

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021237" target="_blank" >RIV/60461373:22330/08:00021237 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture

Popis výsledku v původním jazyce

The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, thespecific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the hig

Název v anglickém jazyce

Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture

Popis výsledku anglicky

The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, thespecific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the hig

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

—

Projekt

<a href="/cs/project/1P05OE189" target="_blank" >1P05OE189: Optimalizace a zavedení technologie vysoce produkčních procesů využívajících rekombinantních methylotrofních kvasinek do průmyslového měřítka</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 13th International Biotechnology Symposium and Exhibition

ISBN

—

ISSN

0168-1656

e-ISSN

—

Počet stran výsledku

2

Strana od-do

—

Název nakladatele

Dalian Institute of Chemical Physics

Místo vydání

Dalian

Místo konání akce

Dalian, China

Datum konání akce

12. 10. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—