Strip-based immunoassay for rapid detection of thiabendazole.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024229" target="_blank" >RIV/60461373:22330/10:00024229 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Strip-based immunoassay for rapid detection of thiabendazole.

Popis výsledku v původním jazyce

This study reports development of an immunostrip test for thiabendazole detection based on indirect competitive principle using carbon particles as a label. Nitrocellulose membrane strip was coated with a thiabendazole-protein conjugate in the defined test zone. In flow of an antibody-carbon complex and thiabendazole along the strip, the intensity of black colour formed in the test line reflected the thiabendazole concentration and semi-quantitative estimation could be carried out visually. The optimized test was accomplished within 10 min and the visual detection limit was achieved 0.25 ngmL-1 of standard sample. Moreover, immunostrip was evaluated quantitatively using scanning densitometry. Based on standard curve, the detection limit of the proposedtest was as low as 0.08?0.03 ngmL-1 with an IC50 value of 0.60?0.08 ngmL-1 and a linear working range of 0.11?4.13 ngmL-1. Results of testing precision, stability, and specificity demonstrated that the assay provided a reliable performan

Název v anglickém jazyce

Strip-based immunoassay for rapid detection of thiabendazole.

Popis výsledku anglicky

This study reports development of an immunostrip test for thiabendazole detection based on indirect competitive principle using carbon particles as a label. Nitrocellulose membrane strip was coated with a thiabendazole-protein conjugate in the defined test zone. In flow of an antibody-carbon complex and thiabendazole along the strip, the intensity of black colour formed in the test line reflected the thiabendazole concentration and semi-quantitative estimation could be carried out visually. The optimized test was accomplished within 10 min and the visual detection limit was achieved 0.25 ngmL-1 of standard sample. Moreover, immunostrip was evaluated quantitatively using scanning densitometry. Based on standard curve, the detection limit of the proposedtest was as low as 0.08?0.03 ngmL-1 with an IC50 value of 0.60?0.08 ngmL-1 and a linear working range of 0.11?4.13 ngmL-1. Results of testing precision, stability, and specificity demonstrated that the assay provided a reliable performan

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F07%2F0618" target="_blank" >GA525/07/0618: Imunoanalýza pesticidů v dětské-kojenecké výživě a v džusech - vývoj rychlých a jednoduchých detekčních metod</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors and Bioelectronics

ISSN

0956-5663

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000277930000020

EID výsledku v databázi Scopus

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