Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-based identification of cultured biphenyl-metabolizing bacteria from contaminated horseradish rhizosphere soil
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43891573" target="_blank" >RIV/60461373:22330/11:43891573 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68378050:_____/11:00368927 RIV/61388963:_____/11:00368927
Výsledek na webu
<a href="http://dx.doi.org/10.1128/AEM.05465-11" target="_blank" >http://dx.doi.org/10.1128/AEM.05465-11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/AEM.05465-11" target="_blank" >10.1128/AEM.05465-11</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-based identification of cultured biphenyl-metabolizing bacteria from contaminated horseradish rhizosphere soil
Popis výsledku v původním jazyce
Bacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated biphenyl (PCB)-contaminated soil vegetated by horseradish. Isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The usage of MALDI Biotyper for the classification of isolates was evaluated and compared to 16S rRNA gene sequence analysis. A wide spectrum of bacteria was isolated, with Arthrobacter, Serratia, Rhodococcus, and Rhizobium being predominant. Arthrobacter isolates also represented the most diverse group. The use of MALDI Biotyper in many cases permitted the identification at the level of species, which was not achieved by 16S rRNA gene sequence analyses. However, some isolates had to be identified by 16S rRNA gene analyses if MALDI Biotyper-based identification was at the level of probable or not reliable identification, usually due to a lack of reference spectra included in the database.
Název v anglickém jazyce
Matrix-Assisted Laser Desorption Ionization (MALDI)-Time of Flight Mass Spectrometry- and MALDI Biotyper-based identification of cultured biphenyl-metabolizing bacteria from contaminated horseradish rhizosphere soil
Popis výsledku anglicky
Bacteria that are able to utilize biphenyl as a sole source of carbon were extracted and isolated from polychlorinated biphenyl (PCB)-contaminated soil vegetated by horseradish. Isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The usage of MALDI Biotyper for the classification of isolates was evaluated and compared to 16S rRNA gene sequence analysis. A wide spectrum of bacteria was isolated, with Arthrobacter, Serratia, Rhodococcus, and Rhizobium being predominant. Arthrobacter isolates also represented the most diverse group. The use of MALDI Biotyper in many cases permitted the identification at the level of species, which was not achieved by 16S rRNA gene sequence analyses. However, some isolates had to be identified by 16S rRNA gene analyses if MALDI Biotyper-based identification was at the level of probable or not reliable identification, usually due to a lack of reference spectra included in the database.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Applied and Environmental Microbiology
ISSN
0099-2240
e-ISSN
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Svazek periodika
77
Číslo periodika v rámci svazku
19
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
9
Strana od-do
6858-6866
Kód UT WoS článku
000295123300016
EID výsledku v databázi Scopus
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