Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894331" target="_blank" >RIV/60461373:22330/12:43894331 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10096-012-1718-3" target="_blank" >http://dx.doi.org/10.1007/s10096-012-1718-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10096-012-1718-3" target="_blank" >10.1007/s10096-012-1718-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome

Popis výsledku v původním jazyce

The molecular diagnosis of pertussis and parapertussis syndromes is based on the detection of insertion sequences (IS) 481 and 1001, respectively. However, these IS are also detected in the genomes of various Bordetella species, such that they are not specific for either B. pertussis or B. parapertussis. Therefore, we screened the genome of recently circulating isolates of Bordetella species to compare the prevalence of IS481, IS1001 and, also IS1002 with previously published data and to sequence all ISdetected. We also investigated whether the numbers of IS481 and IS1001 copies vary in recently circulating isolates of the different Bordetella species. We used the polymerase chain reaction (PCR) method for screening the genome of circulating isolatesand to prepare the fragments for sequencing. We used Southern blotting and quantitative real-time PCR for quantification of the numbers of IS. We found no significant diversity in the sequences of the IS harboured in the genomes of the Bo

Název v anglickém jazyce

Insertion sequences shared by Bordetella species and implications for the biological diagnosis of pertussis syndrome

Popis výsledku anglicky

The molecular diagnosis of pertussis and parapertussis syndromes is based on the detection of insertion sequences (IS) 481 and 1001, respectively. However, these IS are also detected in the genomes of various Bordetella species, such that they are not specific for either B. pertussis or B. parapertussis. Therefore, we screened the genome of recently circulating isolates of Bordetella species to compare the prevalence of IS481, IS1001 and, also IS1002 with previously published data and to sequence all ISdetected. We also investigated whether the numbers of IS481 and IS1001 copies vary in recently circulating isolates of the different Bordetella species. We used the polymerase chain reaction (PCR) method for screening the genome of circulating isolatesand to prepare the fragments for sequencing. We used Southern blotting and quantitative real-time PCR for quantification of the numbers of IS. We found no significant diversity in the sequences of the IS harboured in the genomes of the Bo

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal Of Clinical Microbiology & Infectious Diseases

ISSN

0934-9723

e-ISSN

—

Svazek periodika

32

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

89-96

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—