Application of direct analysis in real time ionization--mass spectrometry (DART-MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F13%3A43896687" target="_blank" >RIV/60461373:22330/13:43896687 - isvavai.cz</a>

Výsledek na webu

<a href="http://download.springer.com/static/pdf/626/art%253A10.1007%252Fs11306-013-0495-z.pdf?auth66=1393423614_631f4f81253f3d9df2bf5ad56b22c80e&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/626/art%253A10.1007%252Fs11306-013-0495-z.pdf?auth66=1393423614_631f4f81253f3d9df2bf5ad56b22c80e&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11306-013-0495-z" target="_blank" >10.1007/s11306-013-0495-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of direct analysis in real time ionization--mass spectrometry (DART-MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud

Popis výsledku v původním jazyce

Metabolomic fingerprinting enabled by ambient mass spectrometry employing a direct analysis in real time (DART) ion source coupled to a medium?high resolution/accurate mass time-of-flight mass spectrometer (TOFMS) was used as a tool for differentiation between chickens fed by feed that contained 5-8 % (w/w) of chicken bone meal (a banned component) and those representing a reference group, i.e. grown otherwise under the same conditions. In the first step, the sample extraction and DART-TOFMS instrumental conditions were optimized to obtain the broadest possible representation of ionizable compounds occurring in the extracts obtained from chicken muscle and feed on which experimental animals were grown. To this end, a simultaneous (all-in-one) extraction procedure was developed employing water and cyclohexane mixture as the extraction solvents. Under these conditions both polar as well as non-polar metabolites were isolated within a single extraction step. In the next step, metabolomic

Název v anglickém jazyce

Application of direct analysis in real time ionization--mass spectrometry (DART-MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud

Popis výsledku anglicky

Metabolomic fingerprinting enabled by ambient mass spectrometry employing a direct analysis in real time (DART) ion source coupled to a medium?high resolution/accurate mass time-of-flight mass spectrometer (TOFMS) was used as a tool for differentiation between chickens fed by feed that contained 5-8 % (w/w) of chicken bone meal (a banned component) and those representing a reference group, i.e. grown otherwise under the same conditions. In the first step, the sample extraction and DART-TOFMS instrumental conditions were optimized to obtain the broadest possible representation of ionizable compounds occurring in the extracts obtained from chicken muscle and feed on which experimental animals were grown. To this end, a simultaneous (all-in-one) extraction procedure was developed employing water and cyclohexane mixture as the extraction solvents. Under these conditions both polar as well as non-polar metabolites were isolated within a single extraction step. In the next step, metabolomic

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/QI91B306" target="_blank" >QI91B306: Nová strategie hodnocení kvality a autenticity potravinových surovin a produktů živočišného původu v řetězci prvovýroba - spotřebitel</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Metabolomics

ISSN

1573-3882

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

545-557

Kód UT WoS článku

000318905100003

EID výsledku v databázi Scopus

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