Peptide mass mapping as an effective tool for historical mortar analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43895696" target="_blank" >RIV/60461373:22330/14:43895696 - isvavai.cz</a>

Výsledek na webu

<a href="http://ac.els-cdn.com/S0950061813008994/1-s2.0-S0950061813008994-main.pdf?_tid=5723656e-97d0-11e3-978f-00000aacb361&acdnat=1392640862_a75bc3432e7e36340526b27722def713" target="_blank" >http://ac.els-cdn.com/S0950061813008994/1-s2.0-S0950061813008994-main.pdf?_tid=5723656e-97d0-11e3-978f-00000aacb361&acdnat=1392640862_a75bc3432e7e36340526b27722def713</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.conbuildmat.2013.09.059" target="_blank" >10.1016/j.conbuildmat.2013.09.059</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Peptide mass mapping as an effective tool for historical mortar analysis

Popis výsledku v původním jazyce

Recently, peptide mass mapping using MALDI?TOF MS (Matrix-Assisted Laser Desorption/Ionization ? Time of Flight Mass Spectrometry) was shown to be an effective tool for protein analysis in such challenging insoluble materials as historical mortars. The first aim of this study was to investigate the influence of ageing on the performance of peptide mass mapping analysis using MALDI?TOF MS. Therefore, analyses were performed on model mortar samples with different compositions after 5 years of ageing. Thesecond goal of this work was to analyse peptide fragments using LC?MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry), which represents a new approach. The use of this method led to obtaining the sequence information that allowed for unambiguous identification of the proteins present. Our study proved the complementarity of both approaches; therefore, their combination is highly beneficial.

Název v anglickém jazyce

Peptide mass mapping as an effective tool for historical mortar analysis

Popis výsledku anglicky

Recently, peptide mass mapping using MALDI?TOF MS (Matrix-Assisted Laser Desorption/Ionization ? Time of Flight Mass Spectrometry) was shown to be an effective tool for protein analysis in such challenging insoluble materials as historical mortars. The first aim of this study was to investigate the influence of ageing on the performance of peptide mass mapping analysis using MALDI?TOF MS. Therefore, analyses were performed on model mortar samples with different compositions after 5 years of ageing. Thesecond goal of this work was to analyse peptide fragments using LC?MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry), which represents a new approach. The use of this method led to obtaining the sequence information that allowed for unambiguous identification of the proteins present. Our study proved the complementarity of both approaches; therefore, their combination is highly beneficial.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Construction and Building Materials

ISSN

0950-0618

e-ISSN

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Svazek periodika

50

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

219-225

Kód UT WoS článku

000330489200026

EID výsledku v databázi Scopus

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