Putative P-1B-type ATPase from the bacterium Achromobacter xylosoxidans A8 alters Pb2 /Zn2 /Cd2 -resistance and accumulation in Saccharomyces cerevisiae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897361" target="_blank" >RIV/60461373:22330/14:43897361 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bbamem.2014.01.023" target="_blank" >http://dx.doi.org/10.1016/j.bbamem.2014.01.023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2014.01.023" target="_blank" >10.1016/j.bbamem.2014.01.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Putative P-1B-type ATPase from the bacterium Achromobacter xylosoxidans A8 alters Pb2 /Zn2 /Cd2 -resistance and accumulation in Saccharomyces cerevisiae

Popis výsledku v původním jazyce

PbtA, a putative P-1B-type ATPase from the Gram-negative soil bacterium Achromobacter xylosoxidans A8 responsible for Pb2+/Zn2+/Cd2+-resistance in Escherichia coli, was heterologously expressed in Saccharomyces cerevisiae. When present in Zn2+- and Pb2+/Cd2+-hypersensitive S. cerevisiae strains CM137 and DTY168, respectively, PbtA was able to restore Zn2+- and Pb2+-resistant phenotype. At the same time, the increase of Pb, Zn, and Cd accumulation in yeast was observed. However, Cd2+-tolerance of the pbtA-bearing yeasts dramatically decreased. The PbtA-eGFP fusion protein was localized primarily in the tonoplast and also in the plasma membrane and the perinuclear region corresponding to the endoplasmic reticulum at later growth stages. This indicates that PbtA protein is successfully incorporated into membranes in yeasts. Since PbtA caused a substantial increase of Pb2+/Zn2+-resistance and accumulation in baker's yeast, we propose its further use for the genetic modification of suitable

Název v anglickém jazyce

Putative P-1B-type ATPase from the bacterium Achromobacter xylosoxidans A8 alters Pb2 /Zn2 /Cd2 -resistance and accumulation in Saccharomyces cerevisiae

Popis výsledku anglicky

PbtA, a putative P-1B-type ATPase from the Gram-negative soil bacterium Achromobacter xylosoxidans A8 responsible for Pb2+/Zn2+/Cd2+-resistance in Escherichia coli, was heterologously expressed in Saccharomyces cerevisiae. When present in Zn2+- and Pb2+/Cd2+-hypersensitive S. cerevisiae strains CM137 and DTY168, respectively, PbtA was able to restore Zn2+- and Pb2+-resistant phenotype. At the same time, the increase of Pb, Zn, and Cd accumulation in yeast was observed. However, Cd2+-tolerance of the pbtA-bearing yeasts dramatically decreased. The PbtA-eGFP fusion protein was localized primarily in the tonoplast and also in the plasma membrane and the perinuclear region corresponding to the endoplasmic reticulum at later growth stages. This indicates that PbtA protein is successfully incorporated into membranes in yeasts. Since PbtA caused a substantial increase of Pb2+/Zn2+-resistance and accumulation in baker's yeast, we propose its further use for the genetic modification of suitable

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-28283S" target="_blank" >GA13-28283S: Spojení ekologie mikrobiálních komunit a degradace xenobiotik - využití metagenomiky k analýze mikrobiálního degradačního potenciálu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

—

Svazek periodika

1838

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

1338-1348

Kód UT WoS článku

000334008100017

EID výsledku v databázi Scopus

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