Development of immunochemical method for the detection of new synthetic cannabinoids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43899087" target="_blank" >RIV/60461373:22330/14:43899087 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of immunochemical method for the detection of new synthetic cannabinoids

Popis výsledku v původním jazyce

Cannabinoids are a class of diverse chemical compounds that act on cannabinoid receptors on cells that repress neurotransmitter release in the brain. The aim of this study was to develop the immunochemical method for the detection of selected synthetic cannabinoids. Two formats of the indirect competitive ELISA were built as combination of conjugate of synthetic cannabinoids with bovine serum albumin (AŠ089-BSA) with polyclonal antibody Ab 197 (with detection limit 9 +- 3 pg/mL), and polyclonal antibodyAb 198 (with detection limit 8 +- 1 pg/mL). The reactivity with structurally or functionally similar substances was tested to verify the specificity of the assembled formats. Antibodies did not interact significantly with the test substances.

Název v anglickém jazyce

Development of immunochemical method for the detection of new synthetic cannabinoids

Popis výsledku anglicky

Cannabinoids are a class of diverse chemical compounds that act on cannabinoid receptors on cells that repress neurotransmitter release in the brain. The aim of this study was to develop the immunochemical method for the detection of selected synthetic cannabinoids. Two formats of the indirect competitive ELISA were built as combination of conjugate of synthetic cannabinoids with bovine serum albumin (AŠ089-BSA) with polyclonal antibody Ab 197 (with detection limit 9 +- 3 pg/mL), and polyclonal antibodyAb 198 (with detection limit 8 +- 1 pg/mL). The reactivity with structurally or functionally similar substances was tested to verify the specificity of the assembled formats. Antibodies did not interact significantly with the test substances.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/VG20122015075" target="_blank" >VG20122015075: Nové syntetické drogy (NSD) - vytvoření komplexní toxikologické databáze, vývoj metodik jejich detekce včetně rychlých imunochemických testů, jejich behaviorální farmakologie, farmakokinetika a biotransformace u potkanů, epidemiologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů