Diversity of O antigens within the genus Cronobacter: from disorder to order

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43900571" target="_blank" >RIV/60461373:22330/15:43900571 - isvavai.cz</a>

Výsledek na webu

<a href="http://aem.asm.org/content/81/16/5574.full" target="_blank" >http://aem.asm.org/content/81/16/5574.full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.00277-15" target="_blank" >10.1128/AEM.00277-15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diversity of O antigens within the genus Cronobacter: from disorder to order

Popis výsledku v původním jazyce

Cronobacter species are Gram-negative opportunistic pathogens that can cause serious infections in neonates. The lipopolysaccharides (LPSs) that form part of the outer membrane of such bacteria are possibly related to the virulence of particular bacterial strains. However, currently there is no clear overview of O-antigen diversity within the various Cronobacter strains and links with virulence. In this study, we tested a total of 82 strains, covering each of the Cronobacter species. The nucleotide variability of the O-antigen gene cluster was determined by restriction fragment length polymorphism (RFLP) analysis. As a result, the 82 strains were distributed into 11 previously published serotypes and 6 new serotypes, each defined by its characteristicrestriction profile. These new serotypes were confirmed using genomic analysis of strains available in public databases: GenBank and Pub-MLST Cronobacter. Laboratory strains were then tested using the current serotype-specific PCR probes.

Název v anglickém jazyce

Diversity of O antigens within the genus Cronobacter: from disorder to order

Popis výsledku anglicky

Cronobacter species are Gram-negative opportunistic pathogens that can cause serious infections in neonates. The lipopolysaccharides (LPSs) that form part of the outer membrane of such bacteria are possibly related to the virulence of particular bacterial strains. However, currently there is no clear overview of O-antigen diversity within the various Cronobacter strains and links with virulence. In this study, we tested a total of 82 strains, covering each of the Cronobacter species. The nucleotide variability of the O-antigen gene cluster was determined by restriction fragment length polymorphism (RFLP) analysis. As a result, the 82 strains were distributed into 11 previously published serotypes and 6 new serotypes, each defined by its characteristicrestriction profile. These new serotypes were confirmed using genomic analysis of strains available in public databases: GenBank and Pub-MLST Cronobacter. Laboratory strains were then tested using the current serotype-specific PCR probes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied and Environmental Microbiology

ISSN

0099-2240

e-ISSN

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Svazek periodika

81

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

5574-5582

Kód UT WoS článku

000358278600032

EID výsledku v databázi Scopus

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