Effect of Fusarium culmorum Tri Gene Transcription on Deoxynivalenol and D3G Levels in Two Different Barley Cultivars
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43900590" target="_blank" >RIV/60461373:22330/15:43900590 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00027006:_____/15:00003254
Výsledek na webu
<a href="http://onlinelibrary.wiley.com/doi/10.1111/jph.12359/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jph.12359/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/jph.12359" target="_blank" >10.1111/jph.12359</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of Fusarium culmorum Tri Gene Transcription on Deoxynivalenol and D3G Levels in Two Different Barley Cultivars
Popis výsledku v původním jazyce
Fusarium culmorum is a phytopathogenic fungus causing Fusarium head blight (FHB), which negatively affects cereals by producing mycotoxins, such as deoxynivalenol (DON). In this work, two barley cultivars, Chevron and Pedant, with different degrees of resistance to FHB were inoculated with F. culmorum. The transcription levels of the Fusarium Tri genes and barley UDP-glycosyltransferase genes were investigated. The amounts of pathogen, DON and the detoxification product deoxynivalenol-3-Oglucoside (D3G)were monitored. The greatest amounts of pathogen were detected at 21 days postinoculation (dpi) and were much lower in cv. Chevron than in cv. Pedant. No differences in the total DON conversion to D3G were observed between the cultivars. Ubiquitin-conjugating enzyme (UBC) was identified and then used as a reference gene to monitor transcription of the Fusarium Tri genes in infected barley. Transcription of the F. culmorum Tri5, Tri4, Tri6 and Tri10 genes differed between the two cultiva
Název v anglickém jazyce
Effect of Fusarium culmorum Tri Gene Transcription on Deoxynivalenol and D3G Levels in Two Different Barley Cultivars
Popis výsledku anglicky
Fusarium culmorum is a phytopathogenic fungus causing Fusarium head blight (FHB), which negatively affects cereals by producing mycotoxins, such as deoxynivalenol (DON). In this work, two barley cultivars, Chevron and Pedant, with different degrees of resistance to FHB were inoculated with F. culmorum. The transcription levels of the Fusarium Tri genes and barley UDP-glycosyltransferase genes were investigated. The amounts of pathogen, DON and the detoxification product deoxynivalenol-3-Oglucoside (D3G)were monitored. The greatest amounts of pathogen were detected at 21 days postinoculation (dpi) and were much lower in cv. Chevron than in cv. Pedant. No differences in the total DON conversion to D3G were observed between the cultivars. Ubiquitin-conjugating enzyme (UBC) was identified and then used as a reference gene to monitor transcription of the Fusarium Tri genes in infected barley. Transcription of the F. culmorum Tri5, Tri4, Tri6 and Tri10 genes differed between the two cultiva
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/QI111B044" target="_blank" >QI111B044: Komplexní strategie pro minimalizaci negativního dopadu infekce toxikogenními houbami r. Fusarium v obilovinách a odvozených produktech.</a><br>
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Phytopathology
ISSN
0931-1785
e-ISSN
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Svazek periodika
163
Číslo periodika v rámci svazku
7-8
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
593-603
Kód UT WoS článku
000357465500011
EID výsledku v databázi Scopus
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