Transformation of raw feather waste into digestible peptides and amino acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901689" target="_blank" >RIV/60461373:22330/16:43901689 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/jctb.4912" target="_blank" >http://dx.doi.org/10.1002/jctb.4912</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jctb.4912" target="_blank" >10.1002/jctb.4912</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transformation of raw feather waste into digestible peptides and amino acids

Popis výsledku v původním jazyce

BACKGROUND: Millions of tons of feather waste from the poultry industry are disposed of annually despite containing a high level of keratin. The aim of this study was to compare the hydrolysis of non-treated feather waste using three different approaches (whole cell microbial digestion, enzymatic and chemical cleavage) and to test the use of hydrolysates as peptone substitutes in a culture medium. RESULTS: Among bacterial isolates, Pseudomonas sp. P5 exhibited the highest keratinolytic activity and efficiency to hydrolyse raw feather material. The hydrolysates contained up to 301 mg L-1 of free amino acids and 6.2 g L-1 of peptides. Hydrolysates obtained by digestion using semi-purified keratinase from Pseudomonas sp. P5 were richer in amino acids (1191 mg L-1, 56% essential ones) but peptides were present in lower amounts (up to 3.3 g L-1). The third approach was feather treatment under mild alkaline conditions. This provided the highest amount of peptides (17.2 g L-1) but a significantly lower level of amino acids, especially the essential ones. CONCLUSIONS: All approaches tested could convert raw feather waste into products of commercial value with proven use in a cultivation medium. The level of peptides, their molecular size and amino acid composition was dependent on the method used.

Název v anglickém jazyce

Transformation of raw feather waste into digestible peptides and amino acids

Popis výsledku anglicky

BACKGROUND: Millions of tons of feather waste from the poultry industry are disposed of annually despite containing a high level of keratin. The aim of this study was to compare the hydrolysis of non-treated feather waste using three different approaches (whole cell microbial digestion, enzymatic and chemical cleavage) and to test the use of hydrolysates as peptone substitutes in a culture medium. RESULTS: Among bacterial isolates, Pseudomonas sp. P5 exhibited the highest keratinolytic activity and efficiency to hydrolyse raw feather material. The hydrolysates contained up to 301 mg L-1 of free amino acids and 6.2 g L-1 of peptides. Hydrolysates obtained by digestion using semi-purified keratinase from Pseudomonas sp. P5 were richer in amino acids (1191 mg L-1, 56% essential ones) but peptides were present in lower amounts (up to 3.3 g L-1). The third approach was feather treatment under mild alkaline conditions. This provided the highest amount of peptides (17.2 g L-1) but a significantly lower level of amino acids, especially the essential ones. CONCLUSIONS: All approaches tested could convert raw feather waste into products of commercial value with proven use in a cultivation medium. The level of peptides, their molecular size and amino acid composition was dependent on the method used.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/TE01020080" target="_blank" >TE01020080: Centrum kompetence pro výzkum biorafinací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chemical Technology and Biotechnology

ISSN

0268-2575

e-ISSN

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Svazek periodika

91

Číslo periodika v rámci svazku

červen

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1629-1637

Kód UT WoS článku

000375768300008

EID výsledku v databázi Scopus

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