Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901693" target="_blank" >RIV/60461373:22330/16:43901693 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22810/16:43901693

Výsledek na webu

<a href="http://dx.doi.org/10.1093/femsle/fnw031" target="_blank" >http://dx.doi.org/10.1093/femsle/fnw031</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsle/fnw031" target="_blank" >10.1093/femsle/fnw031</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation

Popis výsledku v původním jazyce

Flow cytometry, in combination with fluorescent staining, was used to evaluate population heterogeneity in acetonebutanol- ethanol fermentation that was carried out with type strain Clostridium beijerinckii NCIMB 8052 and non-type C. pasteurianum NRRL B-598. A combination of propidium iodide (PI) and carboxyfluorescein diacetate (CFDA), PI plus Syto-9 and bis-oxonol (BOX) alone were employed to distinguish between active and damaged cells together with simultaneous detection of spores. These strategies provided valuable information on the physiological state of clostridia. CFDA and PI staining gave the best separation of four distinct subpopulations of enzymatically active cells, doubly stained cells, damaged cells and spores. Proportional representation of cells in particular sub-regions correlated with growth characteristics, fermentation parameters such as substrate consumption and product formation in both species under different cultivation conditions.

Název v anglickém jazyce

Evaluation of viability, metabolic activity and spore quantity in clostridial cultures during ABE fermentation

Popis výsledku anglicky

Flow cytometry, in combination with fluorescent staining, was used to evaluate population heterogeneity in acetonebutanol- ethanol fermentation that was carried out with type strain Clostridium beijerinckii NCIMB 8052 and non-type C. pasteurianum NRRL B-598. A combination of propidium iodide (PI) and carboxyfluorescein diacetate (CFDA), PI plus Syto-9 and bis-oxonol (BOX) alone were employed to distinguish between active and damaged cells together with simultaneous detection of spores. These strategies provided valuable information on the physiological state of clostridia. CFDA and PI staining gave the best separation of four distinct subpopulations of enzymatically active cells, doubly stained cells, damaged cells and spores. Proportional representation of cells in particular sub-regions correlated with growth characteristics, fermentation parameters such as substrate consumption and product formation in both species under different cultivation conditions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/TE01020080" target="_blank" >TE01020080: Centrum kompetence pro výzkum biorafinací</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS MICROBIOLOGY LETTERS

ISSN

0378-1097

e-ISSN

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Svazek periodika

363

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

000377967000004

EID výsledku v databázi Scopus

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