Synergistic processing of biphenyl and benzoate: Carbon flow through the bacterial community in polychlorinated-biphenyl-contaminated soil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901733" target="_blank" >RIV/60461373:22330/16:43901733 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/srep22145" target="_blank" >http://dx.doi.org/10.1038/srep22145</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/srep22145" target="_blank" >10.1038/srep22145</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Synergistic processing of biphenyl and benzoate: Carbon flow through the bacterial community in polychlorinated-biphenyl-contaminated soil

Popis výsledku v původním jazyce

Polychlorinated biphenyls (PCBs) are toxic and persistent organic pollutants that can be transformed to varying extents by some microorganisms in soil. The aerobic mineralization of PCBs involves the upper (biphenyl, BP) and lower (benzoate, BZ) pathways, but interactions between members of the microbial community active throughout the stages of PCB biodegradation are not well understood. This study investigates BP and BZ biodegradation and subsequent carbon flow between these and other guilds in PCB-contaminated soil from Kodiak, Alaska. DNA stable isotope probing (SIP) was used to identify members of the bacterial community involved in utilization of 13C-biphenyl (an analogue of PCBs), and 13C-benzoate (a product/intermediate of biphenyl degradation and analogue of chlorobenzoates). By performing SIP in parallel with these two substrates over a time course, we reveal microbes performing the upper (BP) and/or lower (BZ) degradation pathways, as well as heterotrophic bacteria involved indirectly in processing of carbon derived from these substrates. Different members of the phyla Actinobacteria and Proteobacteria dominated the biotransformation of BP and BZ in soil. Substrate mineralization rates and shifts in relative abundance and phylogenetic identity of labeled organisms suggest that BP and BZ biotransformations were performed by microorganisms with different growth strategies: BZ-associated bacteria were fast growing, potentially copiotrophic organisms, while microbes that transform BP were oliotrophic, slower growing, organisms. Overall, our findings provide novel insight into the functional interactions of soil bacterial community members active in processing biphenyl and related aromatic compounds in soil, and reveal how carbon flows through a bacterial community.

Název v anglickém jazyce

Synergistic processing of biphenyl and benzoate: Carbon flow through the bacterial community in polychlorinated-biphenyl-contaminated soil

Popis výsledku anglicky

Polychlorinated biphenyls (PCBs) are toxic and persistent organic pollutants that can be transformed to varying extents by some microorganisms in soil. The aerobic mineralization of PCBs involves the upper (biphenyl, BP) and lower (benzoate, BZ) pathways, but interactions between members of the microbial community active throughout the stages of PCB biodegradation are not well understood. This study investigates BP and BZ biodegradation and subsequent carbon flow between these and other guilds in PCB-contaminated soil from Kodiak, Alaska. DNA stable isotope probing (SIP) was used to identify members of the bacterial community involved in utilization of 13C-biphenyl (an analogue of PCBs), and 13C-benzoate (a product/intermediate of biphenyl degradation and analogue of chlorobenzoates). By performing SIP in parallel with these two substrates over a time course, we reveal microbes performing the upper (BP) and/or lower (BZ) degradation pathways, as well as heterotrophic bacteria involved indirectly in processing of carbon derived from these substrates. Different members of the phyla Actinobacteria and Proteobacteria dominated the biotransformation of BP and BZ in soil. Substrate mineralization rates and shifts in relative abundance and phylogenetic identity of labeled organisms suggest that BP and BZ biotransformations were performed by microorganisms with different growth strategies: BZ-associated bacteria were fast growing, potentially copiotrophic organisms, while microbes that transform BP were oliotrophic, slower growing, organisms. Overall, our findings provide novel insight into the functional interactions of soil bacterial community members active in processing biphenyl and related aromatic compounds in soil, and reveal how carbon flows through a bacterial community.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

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Svazek periodika

6

Číslo periodika v rámci svazku

FEB 26 2016

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

22145

Kód UT WoS článku

000370864100004

EID výsledku v databázi Scopus

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