Diversity changes of bacterial populations in soil contaminated by chlorinated phenols, dioxins and furans

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Diversity changes of bacterial populations in soil contaminated by chlorinated phenols, dioxins and furans

Popis výsledku v původním jazyce

Remediation of contaminated soils with highly chlorinated aromatic compounds has been challenging. These contaminants are very persistent, toxic for living organisms, and have a low bioavailability. Physicochemical treatments for their removal are costly, energy demanding and not environmental friendly. Therefore, cheaper alternatives are sought; with bioremediation being a promising option. The aim of this project was to monitor the diversity of bacterial communities in soil from the surroundings of a wood impregnation facility contaminated by chlorophenols, dioxins, and furans. The soil was remediated using a combination of different strategies, including different energy milling, addition of activated sludge, and anaerobic conditions in bioreactors for several months. Soil samples were collected at each step of the bioremediation process and metagenomic DNA was isolated from all samples including the original contaminated soil samples. Bacterial diversity was analyzed through pyrosequencing of 16S rRNA gene amplicons. Furthermore, functional genes potentially responsible for the dehalogenation of chlorophenols were monitored through qPCR in all samples. The results provide information on the dynamics of the bacterial communities over time and under different treatments. Such information may be useful for further bioremediation projects and for understanding the major drivers of bacterial community structure.

Název v anglickém jazyce

Diversity changes of bacterial populations in soil contaminated by chlorinated phenols, dioxins and furans

Popis výsledku anglicky

Remediation of contaminated soils with highly chlorinated aromatic compounds has been challenging. These contaminants are very persistent, toxic for living organisms, and have a low bioavailability. Physicochemical treatments for their removal are costly, energy demanding and not environmental friendly. Therefore, cheaper alternatives are sought; with bioremediation being a promising option. The aim of this project was to monitor the diversity of bacterial communities in soil from the surroundings of a wood impregnation facility contaminated by chlorophenols, dioxins, and furans. The soil was remediated using a combination of different strategies, including different energy milling, addition of activated sludge, and anaerobic conditions in bioreactors for several months. Soil samples were collected at each step of the bioremediation process and metagenomic DNA was isolated from all samples including the original contaminated soil samples. Bacterial diversity was analyzed through pyrosequencing of 16S rRNA gene amplicons. Furthermore, functional genes potentially responsible for the dehalogenation of chlorophenols were monitored through qPCR in all samples. The results provide information on the dynamics of the bacterial communities over time and under different treatments. Such information may be useful for further bioremediation projects and for understanding the major drivers of bacterial community structure.

Druh

D - Stať ve sborníku

CEP obor

DK - Kontaminace a dekontaminace půdy včetně pesticidů

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 4th International Conference on Chemical Technology

ISBN

978-80-86238-94-4

ISSN

2336-811X

e-ISSN

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Počet stran výsledku

6

Strana od-do

493-498

Název nakladatele

Czech Society of Industrial Chemistry

Místo vydání

Praha

Místo konání akce

Mikulov

Datum konání akce

25. 4. 2016

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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