Carboxylic Carbon Quantum Dots as a Fluorescent Sensing Platform for DNA Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901908" target="_blank" >RIV/60461373:22330/16:43901908 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/16:43901908

Výsledek na webu

<a href="http://pubs.acs.org/doi/abs/10.1021/acsami.5b10160" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/acsami.5b10160</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.5b10160" target="_blank" >10.1021/acsami.5b10160</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Carboxylic Carbon Quantum Dots as a Fluorescent Sensing Platform for DNA Detection

Popis výsledku v původním jazyce

The demand for simple, sensitive, affordable, and selective DNA biosensors is ubiquitous, due to the important role that DNA detection performs in the areas of disease diagnostics, environment monitoring, and food safety. A novel application of carboxylic carbon quantum dots (cCQD) is highlighted in this study. Herein, cCQD function as a nanoquencher in the detection of nucleic acid based on a homogeneous fluorescent assay. To that purpose, the performance of two types of cCQD, namely, citric acid QD and malic acid QD, is evaluated. The principle behind the sensing of nucleic acid lies in the different propensity of single stranded DNA and double -stranded DNA to adsorb onto the surface of cCQD. For both types of cCQD, a superior range of detection of at least 3 orders of magnitude is achieved, and the potential to distinguish single-base mismatch is also exhibited. These findings are anticipated to provide valuable insights on the employment of cCQD for the fabrication of future DNA biosensors.

Název v anglickém jazyce

Carboxylic Carbon Quantum Dots as a Fluorescent Sensing Platform for DNA Detection

Popis výsledku anglicky

The demand for simple, sensitive, affordable, and selective DNA biosensors is ubiquitous, due to the important role that DNA detection performs in the areas of disease diagnostics, environment monitoring, and food safety. A novel application of carboxylic carbon quantum dots (cCQD) is highlighted in this study. Herein, cCQD function as a nanoquencher in the detection of nucleic acid based on a homogeneous fluorescent assay. To that purpose, the performance of two types of cCQD, namely, citric acid QD and malic acid QD, is evaluated. The principle behind the sensing of nucleic acid lies in the different propensity of single stranded DNA and double -stranded DNA to adsorb onto the surface of cCQD. For both types of cCQD, a superior range of detection of at least 3 orders of magnitude is achieved, and the potential to distinguish single-base mismatch is also exhibited. These findings are anticipated to provide valuable insights on the employment of cCQD for the fabrication of future DNA biosensors.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CA - Anorganická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-09001S" target="_blank" >GA15-09001S: Chemické modifikace materiálů na bázi grafenu: Syntéza grafanu a halogengrafenu</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Applied Materials and Interfaces

ISSN

1944-8244

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1951-1957

Kód UT WoS článku

000369044100049

EID výsledku v databázi Scopus

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