New findings in potential applications of tobacco osmotin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43902015" target="_blank" >RIV/60461373:22330/16:43902015 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.pep.2016.09.008" target="_blank" >http://dx.doi.org/10.1016/j.pep.2016.09.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pep.2016.09.008" target="_blank" >10.1016/j.pep.2016.09.008</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New findings in potential applications of tobacco osmotin

Popis výsledku v původním jazyce

The osmotin protein is involved in both monocot and dicot plant responses to biotic and abiotic stress. To determine the biological activity of osmotin, the gene was amplified from tobacco genomic DNA, fused with the hexahistidine tag motif and successfully expressed in Escherichia coli, after which the recombinant osmotin was purified and renatured. Various activities were then tested, including hemolytic activity, toxicity against human embryonic kidney cells, and the antifungal activity of the recombinant osmotin. We found that osmotin had no adverse effects on human kidney cells up to a concentration of 500 mu g.ml(-1). However, the purified osmotin also had significant antimicrobial activity, specifically against fungal pathogens causing candidiasis and otitis, and against the common food pathogens.

Název v anglickém jazyce

New findings in potential applications of tobacco osmotin

Popis výsledku anglicky

The osmotin protein is involved in both monocot and dicot plant responses to biotic and abiotic stress. To determine the biological activity of osmotin, the gene was amplified from tobacco genomic DNA, fused with the hexahistidine tag motif and successfully expressed in Escherichia coli, after which the recombinant osmotin was purified and renatured. Various activities were then tested, including hemolytic activity, toxicity against human embryonic kidney cells, and the antifungal activity of the recombinant osmotin. We found that osmotin had no adverse effects on human kidney cells up to a concentration of 500 mu g.ml(-1). However, the purified osmotin also had significant antimicrobial activity, specifically against fungal pathogens causing candidiasis and otitis, and against the common food pathogens.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-22276S" target="_blank" >GA15-22276S: Příprava transgenních rostlin se zvýšenou rezistencí proti stresu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protein Expression and Purification

ISSN

1046-5928

e-ISSN

—

Svazek periodika

129

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

84-93

Kód UT WoS článku

000386194500011

EID výsledku v databázi Scopus

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