Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43902651" target="_blank" >RIV/60461373:22330/17:43902651 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11696-017-0141-8" target="_blank" >https://link.springer.com/article/10.1007/s11696-017-0141-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11696-017-0141-8" target="_blank" >10.1007/s11696-017-0141-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

Popis výsledku v původním jazyce

Emerging resistance of microbial cells towards antibiotics or disinfectants leads to an increase of nosocomial diseases, often biofilm related, and gives rise to the need for research for new antimicrobial substances. These studies are often focused on substances that are able to support the efficacy of currently used antimicrobials, reduce their required dosage and reduce the probability of resistance development. This work brings new insights into the in vitro interaction of rhamnolipid biosurfactant and amphotericin B against Candida sp. or Trichosporon sp.. The minimum inhibitory concentrations and fractional inhibitory concentration indexes for both planktonic and biofilm cells were determined by the chequerboard microdilution method. Combination effect of rhamnolipid and amphotericin B was observed for both yeasts. Synergy, defined as a fractional inhibitory concentration (FICi) index of 0.50, was observed in both planktonic and biofilm cells of Trichosporon cutaneum. For C. parapsilosis, synergistic effect (FICi 0.5) was observed for planktonic cells, and additive effect (FICi 0.8) for biofilm cells. The influence of concentrations established as FICi on biofilm formation and susceptibility was studied by light microscopy. The highest inhibition (90% colonized area reduction) of initial adhesion of C. parapsilosis was observed when biofilm FIC was applied.

Název v anglickém jazyce

Synergistic action of amphotericin B and rhamnolipid in combination on Candida parapsilosis and Trichosporon cutaneum

Popis výsledku anglicky

Emerging resistance of microbial cells towards antibiotics or disinfectants leads to an increase of nosocomial diseases, often biofilm related, and gives rise to the need for research for new antimicrobial substances. These studies are often focused on substances that are able to support the efficacy of currently used antimicrobials, reduce their required dosage and reduce the probability of resistance development. This work brings new insights into the in vitro interaction of rhamnolipid biosurfactant and amphotericin B against Candida sp. or Trichosporon sp.. The minimum inhibitory concentrations and fractional inhibitory concentration indexes for both planktonic and biofilm cells were determined by the chequerboard microdilution method. Combination effect of rhamnolipid and amphotericin B was observed for both yeasts. Synergy, defined as a fractional inhibitory concentration (FICi) index of 0.50, was observed in both planktonic and biofilm cells of Trichosporon cutaneum. For C. parapsilosis, synergistic effect (FICi 0.5) was observed for planktonic cells, and additive effect (FICi 0.8) for biofilm cells. The influence of concentrations established as FICi on biofilm formation and susceptibility was studied by light microscopy. The highest inhibition (90% colonized area reduction) of initial adhesion of C. parapsilosis was observed when biofilm FIC was applied.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40105 - Horticulture, viticulture

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Papers

ISSN

0366-6352

e-ISSN

—

Svazek periodika

71

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

10

Strana od-do

1471-1480

Kód UT WoS článku

000405694900010

EID výsledku v databázi Scopus

—