Tertiary structure prediction of potential efflux-pump protein in Clostridium Beijerinckii NRRL B-598

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43913813" target="_blank" >RIV/60461373:22330/17:43913813 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf" target="_blank" >http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tertiary structure prediction of potential efflux-pump protein in Clostridium Beijerinckii NRRL B-598

Popis výsledku v původním jazyce

The fields of computational biochemistry and structural biology are growing rapidly. With various computational tools the whole workflow for in silico modeling of three-dimensional protein structures can be compiled. Here, we present a method for tertiary structure prediction for arcB1, a putative efflux pump gene, recently identified in the genomic sequence of Clostridium beijerinckii NRRL B-598. The strain C. beijerinckii NRRL B-598, formerly misidentified as C. pasteurianum, is an oxygen tolerant heterofermentative anaerobe with the ability of acetone-butanol fermentation, with butanol as the main product. Unfortunately, the mechanism of butanol efflux remains unknown. The translated AcrB1 protein sequence had a high similarity to the already described efflux-pump of Escherichia coli. To study these sequences in a structural context, we constructed a homology model for C. beijerinckii NRRL B-598 and compared it with the corresponding experimentally determined structure from E. coli. The predicted protein model helps us to understand its function and confirm that acrB1 is a gene for nonspecific efflux pump ? a potential pump capable of butanol efflux.

Název v anglickém jazyce

Tertiary structure prediction of potential efflux-pump protein in Clostridium Beijerinckii NRRL B-598

Popis výsledku anglicky

The fields of computational biochemistry and structural biology are growing rapidly. With various computational tools the whole workflow for in silico modeling of three-dimensional protein structures can be compiled. Here, we present a method for tertiary structure prediction for arcB1, a putative efflux pump gene, recently identified in the genomic sequence of Clostridium beijerinckii NRRL B-598. The strain C. beijerinckii NRRL B-598, formerly misidentified as C. pasteurianum, is an oxygen tolerant heterofermentative anaerobe with the ability of acetone-butanol fermentation, with butanol as the main product. Unfortunately, the mechanism of butanol efflux remains unknown. The translated AcrB1 protein sequence had a high similarity to the already described efflux-pump of Escherichia coli. To study these sequences in a structural context, we constructed a homology model for C. beijerinckii NRRL B-598 and compared it with the corresponding experimentally determined structure from E. coli. The predicted protein model helps us to understand its function and confirm that acrB1 is a gene for nonspecific efflux pump ? a potential pump capable of butanol efflux.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-00551S" target="_blank" >GA17-00551S: Vztah mezi efluxem butanolu a tolerancí k butanolu u klostridií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 5th International Conference on Chemical Technology

ISBN

978-80-86238-65-4

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

26-30

Název nakladatele

Česká společnost průmyslové chemie (ČSPCH)

Místo vydání

Praha

Místo konání akce

Mikulov

Datum konání akce

10. 4. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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