Measurement of protein and sugar consumed by bumblebee larvae under standard and food stress conditions using lanthanide complexes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43917202" target="_blank" >RIV/60461373:22330/18:43917202 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/18:43917202 RIV/61388963:_____/19:00505473 RIV/00023272:_____/19:10134443 RIV/00216208:11310/19:10401001 RIV/26296080:_____/19:N0000119

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00040-018-00681-w" target="_blank" >https://link.springer.com/article/10.1007%2Fs00040-018-00681-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00040-018-00681-w" target="_blank" >10.1007/s00040-018-00681-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Measurement of protein and sugar consumed by bumblebee larvae under standard and food stress conditions using lanthanide complexes

Popis výsledku v původním jazyce

Measurement of food consumed by larvae of progressive provisioning bees requires an experimental approach that marks the food sources appropriately. This paper aims to measure the amount of sugars and proteins consumed by a single bumblebee larva and to define, how the proportion between protein and sugar changes under stress conditions. We marked sugar and pollen sources using lanthanide (gadolinium, dysprosium) complexes with diethylene-triamine-pentaacetic acid (DTPA) chelator. We precisely quantified the amount of Gd and Dy in feces, and thus quantified linearly correlated protein and sugar consumed by males and females (workers) of Bombus terrestris during their development. We compared body mass, total amount of ingested sugar and total amount of ingested protein in colonies which are fed ad libitum and in colonies with restricted feeding. Males fed ad libitum during their development ingested 0.52 mg of protein and 4.43 mg of sugar, and workers fed ad libitum ingested 0.54 mg of protein and 4.26 mg of sugar per 1 mg of dry body weight on average. Food-stressed workers ingested 0.51 mg of protein and 4.65 mg of sugar per 1 mg of dry body weight on average. Strong positive correlation between body mass, total protein ingested, and total sugar ingested in all our experiments was present. However, the consumption of sugar grew steeper with the consumption of protein in colonies under food stress compared to the ad libitum-fed colonies. In conclusion, we show the existence of different patterns in ingestion of protein and sugar between well-fed and food-stressed bumblebee colonies.

Název v anglickém jazyce

Measurement of protein and sugar consumed by bumblebee larvae under standard and food stress conditions using lanthanide complexes

Popis výsledku anglicky

Measurement of food consumed by larvae of progressive provisioning bees requires an experimental approach that marks the food sources appropriately. This paper aims to measure the amount of sugars and proteins consumed by a single bumblebee larva and to define, how the proportion between protein and sugar changes under stress conditions. We marked sugar and pollen sources using lanthanide (gadolinium, dysprosium) complexes with diethylene-triamine-pentaacetic acid (DTPA) chelator. We precisely quantified the amount of Gd and Dy in feces, and thus quantified linearly correlated protein and sugar consumed by males and females (workers) of Bombus terrestris during their development. We compared body mass, total amount of ingested sugar and total amount of ingested protein in colonies which are fed ad libitum and in colonies with restricted feeding. Males fed ad libitum during their development ingested 0.52 mg of protein and 4.43 mg of sugar, and workers fed ad libitum ingested 0.54 mg of protein and 4.26 mg of sugar per 1 mg of dry body weight on average. Food-stressed workers ingested 0.51 mg of protein and 4.65 mg of sugar per 1 mg of dry body weight on average. Strong positive correlation between body mass, total protein ingested, and total sugar ingested in all our experiments was present. However, the consumption of sugar grew steeper with the consumption of protein in colonies under food stress compared to the ad libitum-fed colonies. In conclusion, we show the existence of different patterns in ingestion of protein and sugar between well-fed and food-stressed bumblebee colonies.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10605 - Developmental biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Insectes Sociaux

ISSN

0020-1812

e-ISSN

—

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

Prosinec 2018

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85057957187