Effect of non-thermal plasma on AHL-dependent QS systems and biofilm formation in Pseudomonas aeruginosa: Difference between non-hospital and clinical isolates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43918477" target="_blank" >RIV/60461373:22330/19:43918477 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10400046

Výsledek na webu

<a href="https://www.researchgate.net/publication/333302995_Effect_of_non-thermal_plasma_on_AHL-dependent_QS_systems_and_biofilm_formation_in_Pseudomonas_aeruginosa_Difference_between_non-hospital_and_clinical_isolates" target="_blank" >https://www.researchgate.net/publication/333302995_Effect_of_non-thermal_plasma_on_AHL-dependent_QS_systems_and_biofilm_formation_in_Pseudomonas_aeruginosa_Difference_between_non-hospital_and_clinical_isolates</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/1.5090451" target="_blank" >10.1063/1.5090451</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of non-thermal plasma on AHL-dependent QS systems and biofilm formation in Pseudomonas aeruginosa: Difference between non-hospital and clinical isolates

Popis výsledku v původním jazyce

Non-thermal plasma (NTP), generated by DC cometary discharge with a metallic grid at atmospheric pressure, was used to eradicate biofilm of Pseudomonas aeruginosa from titanium alloy Ti-6Al-4V carriers. The NTP exposure may reduce the ability of signal molecules to cause quorum sensing (QS) response in P. aeruginosa and as a result, affect the production of virulence factors including biofilm formation. Two groups of P. aeruginosa were compared, namely strains isolated from the environment (non-hospital) and clinical isolates from hospital environment. For the non-hospital strains, the inhibition of QS systems, mediated mainly by N-acyl-homoserine lactone (AHL) signals, and decrease of total biofilm biomass were observed after the NTP treatment. After 60 minutes of exposure, almost complete eradication of biofilm (54 - 98%) was achieved. Compared to that, the NTP effect on the AHL levels was more pronounced in clinical isolates of P. aeruginosa, but eradication of the biofilm was not achieved. We conclude that NTP affected the AHL-dependent QS systems (las and rhl), which does not necessarily have to result in the regulation of virulence in clinical isolates. (C) 2019 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Název v anglickém jazyce

Effect of non-thermal plasma on AHL-dependent QS systems and biofilm formation in Pseudomonas aeruginosa: Difference between non-hospital and clinical isolates

Popis výsledku anglicky

Non-thermal plasma (NTP), generated by DC cometary discharge with a metallic grid at atmospheric pressure, was used to eradicate biofilm of Pseudomonas aeruginosa from titanium alloy Ti-6Al-4V carriers. The NTP exposure may reduce the ability of signal molecules to cause quorum sensing (QS) response in P. aeruginosa and as a result, affect the production of virulence factors including biofilm formation. Two groups of P. aeruginosa were compared, namely strains isolated from the environment (non-hospital) and clinical isolates from hospital environment. For the non-hospital strains, the inhibition of QS systems, mediated mainly by N-acyl-homoserine lactone (AHL) signals, and decrease of total biofilm biomass were observed after the NTP treatment. After 60 minutes of exposure, almost complete eradication of biofilm (54 - 98%) was achieved. Compared to that, the NTP effect on the AHL levels was more pronounced in clinical isolates of P. aeruginosa, but eradication of the biofilm was not achieved. We conclude that NTP affected the AHL-dependent QS systems (las and rhl), which does not necessarily have to result in the regulation of virulence in clinical isolates. (C) 2019 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA17-15936S" target="_blank" >GA17-15936S: Interakce nanočástic modifikovaných přírodními látkami s biofilmy patogenních mikroorganismů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

AIP Advances

ISSN

2158-3226

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

000477701000050

EID výsledku v databázi Scopus

2-s2.0-85066151036