SIGNALING PATHWAY FOR BUTANOL PRODUCTION IN CLOSTRIDIUM BEIJERINCKII NRRL B-598

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43918863" target="_blank" >RIV/60461373:22330/19:43918863 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/19:PU133862

Výsledek na webu

<a href="https://link.springer.com/article/10.1186/s12864-018-4805-8" target="_blank" >https://link.springer.com/article/10.1186/s12864-018-4805-8</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

SIGNALING PATHWAY FOR BUTANOL PRODUCTION IN CLOSTRIDIUM BEIJERINCKII NRRL B-598

Popis výsledku v původním jazyce

The unstable price of oil in the last decade is the reason for an increased interest in the sustainable production of biofuels. Microbial production is again becoming a competitive way of generating butanol, and it is a suitable alternative to the chemical production of oil. Still, there is plenty of room for the development of highly efficient phenotypes of various solvent-producing organisms. The key lies in the utilisation of systems biology and its models that can help us to fully understand the whole process on a molecular level in the selected organism, and to identify the genomic regions responsible for the desired phenotype features. Here, we present a dynamic model of butanol production in a promising solvent producer, Clostridium beijerinckii NRRL B-598. We utilised a wide range of data we collected over the last two years, including RNA-Seq gene expression, cell viability measured with flow cytometry, and metabolic activity measured with HPLC, to reconstruct a pathway involved in butanol production. Although solventogenic species are extensively studied organisms, we bring the first pathway of the strain C. beijerinckii NRRL B-598. We used an open platform, Cell Collective (cellcollective.org), where the model is publicly available under the name ‘Signalling Pathway for Butanol Production in Clostridium beijerinckii NRRL B-598’, version 1.1. To determine the properties of the pathway, we performed a series of static and dynamic analyses. The presented pathway is an ideal approach for exploring the possibilities of biofuel overproduction as it enables the activation or inactivation of individual genes and metabolites involved in the production of butanol. Not only can the acquired knowledge help with future engineering of the strain, but the results can be generalised for the microbial production of butanol in related species.

Název v anglickém jazyce

SIGNALING PATHWAY FOR BUTANOL PRODUCTION IN CLOSTRIDIUM BEIJERINCKII NRRL B-598

Popis výsledku anglicky

The unstable price of oil in the last decade is the reason for an increased interest in the sustainable production of biofuels. Microbial production is again becoming a competitive way of generating butanol, and it is a suitable alternative to the chemical production of oil. Still, there is plenty of room for the development of highly efficient phenotypes of various solvent-producing organisms. The key lies in the utilisation of systems biology and its models that can help us to fully understand the whole process on a molecular level in the selected organism, and to identify the genomic regions responsible for the desired phenotype features. Here, we present a dynamic model of butanol production in a promising solvent producer, Clostridium beijerinckii NRRL B-598. We utilised a wide range of data we collected over the last two years, including RNA-Seq gene expression, cell viability measured with flow cytometry, and metabolic activity measured with HPLC, to reconstruct a pathway involved in butanol production. Although solventogenic species are extensively studied organisms, we bring the first pathway of the strain C. beijerinckii NRRL B-598. We used an open platform, Cell Collective (cellcollective.org), where the model is publicly available under the name ‘Signalling Pathway for Butanol Production in Clostridium beijerinckii NRRL B-598’, version 1.1. To determine the properties of the pathway, we performed a series of static and dynamic analyses. The presented pathway is an ideal approach for exploring the possibilities of biofuel overproduction as it enables the activation or inactivation of individual genes and metabolites involved in the production of butanol. Not only can the acquired knowledge help with future engineering of the strain, but the results can be generalised for the microbial production of butanol in related species.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

20901 - Industrial biotechnology

Projekt

<a href="/cs/project/GA17-00551S" target="_blank" >GA17-00551S: Vztah mezi efluxem butanolu a tolerancí k butanolu u klostridií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the 7th International Conference on Chemical Technology

ISBN

978-80-88307-00-6

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

46-52

Název nakladatele

Czech Society of Industrial Chemistry

Místo vydání

Prague

Místo konání akce

Mikulov

Datum konání akce

15. 4. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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