Novel Potential Probiotic Lactobacilli for Prevention and Treatment of Vulvovaginal Infections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43920823" target="_blank" >RIV/60461373:22330/20:43920823 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/21:10425690 RIV/00064165:_____/21:10425690

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12602-020-09675-2#citeas" target="_blank" >https://link.springer.com/article/10.1007/s12602-020-09675-2#citeas</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12602-020-09675-2" target="_blank" >10.1007/s12602-020-09675-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel Potential Probiotic Lactobacilli for Prevention and Treatment of Vulvovaginal Infections

Popis výsledku v původním jazyce

Lactobacilli in the vaginal tract are essential to protect against microbial infections. We therefore focused on isolating vaginal lactobacilli from pregnant women and testing their functional properties. Lactobacilli were isolated from 50 vaginal swabs and the purified isolates were identified by MALDI-TOF MS. Functional properties (antimicrobial activity, organic acids and hydrogen peroxide production, antibiotic susceptibility, auto-aggregation, and hydrophobicity) of selected isolates were tested. Lactobacilli (41 strains) were identified in 58% of swabs with a predominance ofLactobacillus crispatus(48%) followed byL. jensenii(21%),L. rhamnosus(14%),L. fermentum(10%), andL. gasseri(7%). The highest antibacterial activity was determined forL. fermentumandL. rhamnosus. Strong anti-Candidaactivity was observed for strainsL. crispatus,L. fermentum, andL. rhamnosus. StrainL. jensenii58C possessed the highest production of hydrogen peroxide (6.32 +/- 0.60 mg/l). The best lactic acid producer was strainL. rhamnosus72A (11.6 +/- 0.2 g/l). All strains were resistant to fluconazole and metronidazole. The highest auto-aggregation was observed for strainL. crispatus51A (98.8 +/- 0.1% after 24 h). StrainL. rhamnosus68A showed the highest hydrophobicity (69.1 +/- 1.4%). StrainsL. fermentumandL. rhamnosusshowed high antibacterial activity and hydrophobicity, and strainsL. crispatuspossessed high auto-aggregation and anti-Candidaactivity. Thus, these strains alone or in a mix could be used for the preparation of probiotic products for treatment and prevention of vulvovaginal infections of pregnant and non-pregnant women.

Název v anglickém jazyce

Novel Potential Probiotic Lactobacilli for Prevention and Treatment of Vulvovaginal Infections

Popis výsledku anglicky

Lactobacilli in the vaginal tract are essential to protect against microbial infections. We therefore focused on isolating vaginal lactobacilli from pregnant women and testing their functional properties. Lactobacilli were isolated from 50 vaginal swabs and the purified isolates were identified by MALDI-TOF MS. Functional properties (antimicrobial activity, organic acids and hydrogen peroxide production, antibiotic susceptibility, auto-aggregation, and hydrophobicity) of selected isolates were tested. Lactobacilli (41 strains) were identified in 58% of swabs with a predominance ofLactobacillus crispatus(48%) followed byL. jensenii(21%),L. rhamnosus(14%),L. fermentum(10%), andL. gasseri(7%). The highest antibacterial activity was determined forL. fermentumandL. rhamnosus. Strong anti-Candidaactivity was observed for strainsL. crispatus,L. fermentum, andL. rhamnosus. StrainL. jensenii58C possessed the highest production of hydrogen peroxide (6.32 +/- 0.60 mg/l). The best lactic acid producer was strainL. rhamnosus72A (11.6 +/- 0.2 g/l). All strains were resistant to fluconazole and metronidazole. The highest auto-aggregation was observed for strainL. crispatus51A (98.8 +/- 0.1% after 24 h). StrainL. rhamnosus68A showed the highest hydrophobicity (69.1 +/- 1.4%). StrainsL. fermentumandL. rhamnosusshowed high antibacterial activity and hydrophobicity, and strainsL. crispatuspossessed high auto-aggregation and anti-Candidaactivity. Thus, these strains alone or in a mix could be used for the preparation of probiotic products for treatment and prevention of vulvovaginal infections of pregnant and non-pregnant women.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PROBIOTICS AND ANTIMICROBIAL PROTEINS

ISSN

1867-1306

e-ISSN

—

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

2020 Jun 24

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000543006100001

EID výsledku v databázi Scopus

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