Antibiofilm agent pterostilbene is able to enhance antibiotics action against Staphylococcus epidermidis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43921091" target="_blank" >RIV/60461373:22330/21:43921091 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.researchgate.net/publication/346411401_Antibiofilm_agent_pterostilbene_is_able_to_enhance_antibiotics_action_against_Staphylococcus_epidermidis" target="_blank" >https://www.researchgate.net/publication/346411401_Antibiofilm_agent_pterostilbene_is_able_to_enhance_antibiotics_action_against_Staphylococcus_epidermidis</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micpath.2020.104632" target="_blank" >10.1016/j.micpath.2020.104632</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antibiofilm agent pterostilbene is able to enhance antibiotics action against Staphylococcus epidermidis

Popis výsledku v původním jazyce

Pterostilbene (PTE) is a naturally occurring compound originally isolated from Pterocarpus spp. It has been widely used in traditional Indian medicine and later discovered to have various beneficial pharmacological effects such as antioxidant properties, hypoglycaemic or antitumor, and antimicrobial activity. This work is focused on demonstrating PTE synergistic effect with erythromycin and tetracycline to reduce their needed effective concentration for suppression of Staphylococcus epidermidis planktonic cells growth and biofilm formation. The secondary aim is to find these combinations effect on the production of its virulence factors. PTE was found to be effective in inhibition of its planktonic cells with MIC80 values 25–37.5 mg l−1. Simultaneously, it decreased the metabolic activity of biofilm cells and was especially effective on a clinical isolate (MBIC80 = 35 mg l−1) in contrast to the conventional antibiotics. In combination, PTE helped the antibiotics to overcome the tolerance of S. epidermidis biofilm cells (5 mg l−1 of each antibiotic with 49 mg l−1 PTE caused more than 85% inhibition of metabolic activity). It permeabilized cytoplasmic membrane of S. epidermidis cells and altered their surface hydrophobicity. Therefore, PTE has a great potential to enhance antibiotics action in the treatment of infections caused by this pathogen.

Název v anglickém jazyce

Antibiofilm agent pterostilbene is able to enhance antibiotics action against Staphylococcus epidermidis

Popis výsledku anglicky

Pterostilbene (PTE) is a naturally occurring compound originally isolated from Pterocarpus spp. It has been widely used in traditional Indian medicine and later discovered to have various beneficial pharmacological effects such as antioxidant properties, hypoglycaemic or antitumor, and antimicrobial activity. This work is focused on demonstrating PTE synergistic effect with erythromycin and tetracycline to reduce their needed effective concentration for suppression of Staphylococcus epidermidis planktonic cells growth and biofilm formation. The secondary aim is to find these combinations effect on the production of its virulence factors. PTE was found to be effective in inhibition of its planktonic cells with MIC80 values 25–37.5 mg l−1. Simultaneously, it decreased the metabolic activity of biofilm cells and was especially effective on a clinical isolate (MBIC80 = 35 mg l−1) in contrast to the conventional antibiotics. In combination, PTE helped the antibiotics to overcome the tolerance of S. epidermidis biofilm cells (5 mg l−1 of each antibiotic with 49 mg l−1 PTE caused more than 85% inhibition of metabolic activity). It permeabilized cytoplasmic membrane of S. epidermidis cells and altered their surface hydrophobicity. Therefore, PTE has a great potential to enhance antibiotics action in the treatment of infections caused by this pathogen.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Pathogenesis

ISSN

0882-4010

e-ISSN

—

Svazek periodika

neuveden

Číslo periodika v rámci svazku

November 2020

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

1-11

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85097192772