Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43923768" target="_blank" >RIV/60461373:22330/21:43923768 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s13353-021-00655-y" target="_blank" >https://link.springer.com/article/10.1007/s13353-021-00655-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13353-021-00655-y" target="_blank" >10.1007/s13353-021-00655-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

Popis výsledku v původním jazyce

Currently, it is clear that the luxS gene has an impact on the process of biofilm formation in Campylobacter jejuni. However, even within the species, naturally occurring strains of Campylobacter lacking the luxS gene exist, which can form biofilms. In order to better understand the genetic determinants and the role of quorum sensing through the LuxS/AI-2 pathway in biofilm formation, a set of mutant/complemented strains of C. jejuni 81–176 were prepared. Additionally, the impact of the mutagenic strategy used against the luxS gene was investigated. Biofilm formation was affected by both the presence and absence of the luxS gene, and by the mutagenic strategy used. Analysis by CLSM showed that all mutant strains formed significantly less biofilm mass when compared to the wild-type. Interestingly, the deletion mutant (∆luxS) showed a larger decrease in biofilm mass than the substitution (∙luxS) and insertional inactivated (: : luxS) mutants, even though all the mutant strains lost the ability to produce autoinducer-2 molecules. Moreover, the biofilm of the ∆luxS mutant lacked the characteristic microcolonies observed in all other strains. The complementation of all mutant strains resulted in restored ability to produce AI-2, to form a complex biofilm, and to develop microcolonies at the level of the wild-type. © 2021, Institute of Plant Genetics, Polish Academy of Sciences, Poznan.

Název v anglickém jazyce

Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

Popis výsledku anglicky

Currently, it is clear that the luxS gene has an impact on the process of biofilm formation in Campylobacter jejuni. However, even within the species, naturally occurring strains of Campylobacter lacking the luxS gene exist, which can form biofilms. In order to better understand the genetic determinants and the role of quorum sensing through the LuxS/AI-2 pathway in biofilm formation, a set of mutant/complemented strains of C. jejuni 81–176 were prepared. Additionally, the impact of the mutagenic strategy used against the luxS gene was investigated. Biofilm formation was affected by both the presence and absence of the luxS gene, and by the mutagenic strategy used. Analysis by CLSM showed that all mutant strains formed significantly less biofilm mass when compared to the wild-type. Interestingly, the deletion mutant (∆luxS) showed a larger decrease in biofilm mass than the substitution (∙luxS) and insertional inactivated (: : luxS) mutants, even though all the mutant strains lost the ability to produce autoinducer-2 molecules. Moreover, the biofilm of the ∆luxS mutant lacked the characteristic microcolonies observed in all other strains. The complementation of all mutant strains resulted in restored ability to produce AI-2, to form a complex biofilm, and to develop microcolonies at the level of the wild-type. © 2021, Institute of Plant Genetics, Polish Academy of Sciences, Poznan.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA18-16549S" target="_blank" >GA18-16549S: Prevalence a diverzita izolátů Campylobacter jejuni a identifikace faktorů umožňujících jejich přežívání</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Applied Genetics

ISSN

1234-1983

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

145-157

Kód UT WoS článku

000689526800001

EID výsledku v databázi Scopus

2-s2.0-85113649391