Fast centrifugal partition chromatography: development of the method for isolation of phytocannabinoids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F22%3A43925453" target="_blank" >RIV/60461373:22330/22:43925453 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fast centrifugal partition chromatography: development of the method for isolation of phytocannabinoids

Popis výsledku v původním jazyce

Fast centrifugal partition chromatography (FCPC) is a preparative chromatography method based on the interaction of two immiscible solvent mixtures. The FCPC is primarily used for the separation and isolation of biologically active compounds. In this work, a new method was developed for the separation and isolation of phytocannabinoids, biologically active compounds of the plant Cannabis sativa L. The primary goal was to separate the positive-acting cannabidiol (CBD) and its precursor, cannabidiol acid (CBDA), from psychotropic compounds of the tetrahydrocannabinol group (Δ9-THC, Δ9-THCA-A) in hemp extract. Based on literature, 38 model mixtures of solvents were selected. Partition coefficients (KD) and separation factors (α) were calculated for each mixture based on the phytocannabinoids presence, which was determined by UHPLC-HRMS. The fallowing system was selected as the most suitable; heptane:ethyl-acetate:ethanol:water (1.5:0.5:1.5:0.5; v:v:v:v), which meet the goal of the work – allow the separation of CBD/CBDA from substances of the tetrahydrocannabinol group. Additionally, this solvent system allows to separate CBD from CBDA, which could be used for the creation of analytical standards or for the further research of biological activity. The purity of CBD and CBDA fractions was finally evaluated by UHPLC-DAD and UHPLCHRMS and the results were discussed with literature

Název v anglickém jazyce

Fast centrifugal partition chromatography: development of the method for isolation of phytocannabinoids

Popis výsledku anglicky

Fast centrifugal partition chromatography (FCPC) is a preparative chromatography method based on the interaction of two immiscible solvent mixtures. The FCPC is primarily used for the separation and isolation of biologically active compounds. In this work, a new method was developed for the separation and isolation of phytocannabinoids, biologically active compounds of the plant Cannabis sativa L. The primary goal was to separate the positive-acting cannabidiol (CBD) and its precursor, cannabidiol acid (CBDA), from psychotropic compounds of the tetrahydrocannabinol group (Δ9-THC, Δ9-THCA-A) in hemp extract. Based on literature, 38 model mixtures of solvents were selected. Partition coefficients (KD) and separation factors (α) were calculated for each mixture based on the phytocannabinoids presence, which was determined by UHPLC-HRMS. The fallowing system was selected as the most suitable; heptane:ethyl-acetate:ethanol:water (1.5:0.5:1.5:0.5; v:v:v:v), which meet the goal of the work – allow the separation of CBD/CBDA from substances of the tetrahydrocannabinol group. Additionally, this solvent system allows to separate CBD from CBDA, which could be used for the creation of analytical standards or for the further research of biological activity. The purity of CBD and CBDA fractions was finally evaluated by UHPLC-DAD and UHPLCHRMS and the results were discussed with literature

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LM2018100" target="_blank" >LM2018100: Infrastruktura pro propagaci metrologie v potravinářství a výživě v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů