Assessing the antimicrobial efficacy of nonthermal plasma treatment on filamentous fungi spores and biofilms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43929370" target="_blank" >RIV/60461373:22330/24:43929370 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/24:43929370 RIV/26722445:_____/24:N0000004 RIV/68407700:21460/24:00374624

Výsledek na webu

<a href="https://doi.org/10.1016/j.foodcont.2024.110522" target="_blank" >https://doi.org/10.1016/j.foodcont.2024.110522</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodcont.2024.110522" target="_blank" >10.1016/j.foodcont.2024.110522</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessing the antimicrobial efficacy of nonthermal plasma treatment on filamentous fungi spores and biofilms

Popis výsledku v původním jazyce

With the increasing resistance of biofilms to existing products and their predominantly negative impact on the environment, efforts are being made to find new alternatives to suppress microbial growth. One of them involves the use of a nonthermal plasma (NTP). This physical method of eradicating microbial growth has been well studied in terms of its application to bacteria and yeasts, while the effect of NTP on microscopic filamentous fungi has been neglected. However, NTP could serve as a viable alternative to existing disinfectants within food industry production facilities to eliminate the development of fungal contamination. This study investigates the effect of a NTP on the metabolic activity of spores regrowth and biofilms of filamentous fungi as well as on the morphological changes of microbial cultures after exposure to NTP. The morphological changes have been imaged using the scanning electron microscopy (SEM). It has been shown that high quality SEM images of the fungal spores can be obtained without a conductive coating, but in case of the fungal biofilms the use of the coating was necessary. In all examined microorganisms, the NTP treatment led to a reduction in the extracellular matrix of the biofilm and inflicted damage to the hyphae. Untreated samples displayed a layered biofilm structure, while post-treatment, the structure transitioned to a sparse, monolayered form. Particularly notable morphological alterations in the biofilm, as evidenced by SEM microscopy, were observed in Alternaria alternata. These changes align with the observed decrease in metabolic activity following NTP treatment, which was most prominent in this strain. © 2024 Elsevier Ltd

Název v anglickém jazyce

Assessing the antimicrobial efficacy of nonthermal plasma treatment on filamentous fungi spores and biofilms

Popis výsledku anglicky

With the increasing resistance of biofilms to existing products and their predominantly negative impact on the environment, efforts are being made to find new alternatives to suppress microbial growth. One of them involves the use of a nonthermal plasma (NTP). This physical method of eradicating microbial growth has been well studied in terms of its application to bacteria and yeasts, while the effect of NTP on microscopic filamentous fungi has been neglected. However, NTP could serve as a viable alternative to existing disinfectants within food industry production facilities to eliminate the development of fungal contamination. This study investigates the effect of a NTP on the metabolic activity of spores regrowth and biofilms of filamentous fungi as well as on the morphological changes of microbial cultures after exposure to NTP. The morphological changes have been imaged using the scanning electron microscopy (SEM). It has been shown that high quality SEM images of the fungal spores can be obtained without a conductive coating, but in case of the fungal biofilms the use of the coating was necessary. In all examined microorganisms, the NTP treatment led to a reduction in the extracellular matrix of the biofilm and inflicted damage to the hyphae. Untreated samples displayed a layered biofilm structure, while post-treatment, the structure transitioned to a sparse, monolayered form. Particularly notable morphological alterations in the biofilm, as evidenced by SEM microscopy, were observed in Alternaria alternata. These changes align with the observed decrease in metabolic activity following NTP treatment, which was most prominent in this strain. © 2024 Elsevier Ltd

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA22-13745S" target="_blank" >GA22-13745S: Zvýšení účinku biokontrolního agens Pythium oligandrum studenou plazmou proti plísňovým biofilmům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FOOD CONTROL

ISSN

0956-7135

e-ISSN

—

Svazek periodika

163

Číslo periodika v rámci svazku

04

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001233240700001

EID výsledku v databázi Scopus

2-s2.0-85190455135