Bimetallic nanoparticle production using Cannabis sativa and Vitis vinifera waste extracts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F24%3A43930567" target="_blank" >RIV/60461373:22330/24:43930567 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2024/ra/d3ra07134k" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2024/ra/d3ra07134k</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d3ra07134k" target="_blank" >10.1039/d3ra07134k</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bimetallic nanoparticle production using Cannabis sativa and Vitis vinifera waste extracts

Popis výsledku v původním jazyce

The utilization of waste materials for the synthesis of nanoparticles has gained significant attention due to its potential for waste valorization and contribution to circular economy. In this study, bimetallic nanoparticles were produced using extracts derived from Cannabis sativa and Vitis vinifera waste, focusing on their green synthesis and antimicrobial activity against Gram-negative bacteria, specifically several strains of Pseudomonas aeruginosa. The Vitis vinifera canes and post-extraction waste from Cannabis sativa were processed using an ethanol extraction method. The extract was then mixed with silver nitrate and tetrachloroauric acid solution at different reagent ratios to optimize the synthesis process. The resulting bimetallic nanoparticles (AgAuNPs) were characterized using UV-vis spectrophotometry, transmission electron microscopy, atomic absorption spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The antimicrobial activity of the biosynthesized AgAuNPs was evaluated against various strains of Pseudomonas aeruginosa. The minimal inhibitory concentration (MIC) was determined using a microcultivation device, and the minimal bactericidal concentration (MBC) was determined through subsequent solid medium cultivation. Additionally, the minimal biofilm inhibitory concentration (MBIC) was assessed using a polystyrene microtiter plate as biofilm carrier and measured through an assay determining the metabolic activity of biofilm cells. The results demonstrated successful synthesis of bimetallic nanoparticles using the extracts from Cannabis sativa and Vitis vinifera waste. The AgAuNPs exhibited significant antimicrobial activity against the tested Pseudomonas aeruginosa strains, inhibiting their growth and biofilm formation. These findings highlight the potential of waste valorization and circular economy in nanoparticle production and their application as effective antimicrobial agents. This study contributes to the growing field of sustainable nanotechnology and provides insights into the utilization of plant waste extracts for the synthesis of bimetallic nanoparticles with antimicrobial properties. The findings support the development of eco-friendly and cost-effective approaches for nanoparticle production while addressing the challenges of waste management and combating microbial infections.

Název v anglickém jazyce

Bimetallic nanoparticle production using Cannabis sativa and Vitis vinifera waste extracts

Popis výsledku anglicky

The utilization of waste materials for the synthesis of nanoparticles has gained significant attention due to its potential for waste valorization and contribution to circular economy. In this study, bimetallic nanoparticles were produced using extracts derived from Cannabis sativa and Vitis vinifera waste, focusing on their green synthesis and antimicrobial activity against Gram-negative bacteria, specifically several strains of Pseudomonas aeruginosa. The Vitis vinifera canes and post-extraction waste from Cannabis sativa were processed using an ethanol extraction method. The extract was then mixed with silver nitrate and tetrachloroauric acid solution at different reagent ratios to optimize the synthesis process. The resulting bimetallic nanoparticles (AgAuNPs) were characterized using UV-vis spectrophotometry, transmission electron microscopy, atomic absorption spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The antimicrobial activity of the biosynthesized AgAuNPs was evaluated against various strains of Pseudomonas aeruginosa. The minimal inhibitory concentration (MIC) was determined using a microcultivation device, and the minimal bactericidal concentration (MBC) was determined through subsequent solid medium cultivation. Additionally, the minimal biofilm inhibitory concentration (MBIC) was assessed using a polystyrene microtiter plate as biofilm carrier and measured through an assay determining the metabolic activity of biofilm cells. The results demonstrated successful synthesis of bimetallic nanoparticles using the extracts from Cannabis sativa and Vitis vinifera waste. The AgAuNPs exhibited significant antimicrobial activity against the tested Pseudomonas aeruginosa strains, inhibiting their growth and biofilm formation. These findings highlight the potential of waste valorization and circular economy in nanoparticle production and their application as effective antimicrobial agents. This study contributes to the growing field of sustainable nanotechnology and provides insights into the utilization of plant waste extracts for the synthesis of bimetallic nanoparticles with antimicrobial properties. The findings support the development of eco-friendly and cost-effective approaches for nanoparticle production while addressing the challenges of waste management and combating microbial infections.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

2046-2069

Svazek periodika

14

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

5309-5318

Kód UT WoS článku

001159065400001

EID výsledku v databázi Scopus

2-s2.0-85184895591