Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932083" target="_blank" >RIV/60461373:22330/25:43932083 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.biorxiv.org/content/10.1101/2024.03.04.583273v1.full" target="_blank" >https://www.biorxiv.org/content/10.1101/2024.03.04.583273v1.full</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome

Popis výsledku v původním jazyce

Many gram-negative bacteria utilize a type III secretion system (T3SS) injectisome totranslocate effector proteins directly into host cells. In Bordetella bronchiseptica, arespiratory pathogen of diverse mammals, the T3SS injectisome contains a uniqueneedle tip filament formed by the Bsp22 protein, which is essential for bacterial persistence in mice. Here, we used B. bronchiseptica and B. pertussis strains with inframe insertions of peptide tags into Bsp22 to investigate their formation using superresolution imaging with fluorophore-labeled nanobodies and biarsenic probes. Onabiotic surfaces, over 50% of bacteria displayed flexible Bsp22 filaments with anaverage length of 1 μm after 3 hours of incubation. Filament growth occurredcontinuously at the distal end. In contrast, during HeLa cell infection, although theT3SS effector BteA was delivered into host cells, and the number of filaments perbacterium remained unchanged, filament length was significantly shorter, averaging0.4 μm after 3 hours of infection. Some Bsp22 filaments formed short physical bridgesbetween bacteria and host cells. During infection of nasal epithelial cells in air-liquidinterface cultures, Bsp22 filaments became scarce, with most bacteria lackingdetectable filaments. This reduction correlated with a specific decline in bsp22 mRNAlevels, while mRNA levels of bscD, encoding the inner membrane ring component ofthe injectisome, remained stable. Our findings demonstrate that Bsp22 synthesis istightly regulated in response to environmental cues, and highlight how filamentcharacteristics of Bordetella T3SS injectisome change in response to differentenvironments.

Název v anglickém jazyce

Assembly and dynamic regulation of the tip filament of the Bordetella type III secretion system injectisome

Popis výsledku anglicky

Many gram-negative bacteria utilize a type III secretion system (T3SS) injectisome totranslocate effector proteins directly into host cells. In Bordetella bronchiseptica, arespiratory pathogen of diverse mammals, the T3SS injectisome contains a uniqueneedle tip filament formed by the Bsp22 protein, which is essential for bacterial persistence in mice. Here, we used B. bronchiseptica and B. pertussis strains with inframe insertions of peptide tags into Bsp22 to investigate their formation using superresolution imaging with fluorophore-labeled nanobodies and biarsenic probes. Onabiotic surfaces, over 50% of bacteria displayed flexible Bsp22 filaments with anaverage length of 1 μm after 3 hours of incubation. Filament growth occurredcontinuously at the distal end. In contrast, during HeLa cell infection, although theT3SS effector BteA was delivered into host cells, and the number of filaments perbacterium remained unchanged, filament length was significantly shorter, averaging0.4 μm after 3 hours of infection. Some Bsp22 filaments formed short physical bridgesbetween bacteria and host cells. During infection of nasal epithelial cells in air-liquidinterface cultures, Bsp22 filaments became scarce, with most bacteria lackingdetectable filaments. This reduction correlated with a specific decline in bsp22 mRNAlevels, while mRNA levels of bscD, encoding the inner membrane ring component ofthe injectisome, remained stable. Our findings demonstrate that Bsp22 synthesis istightly regulated in response to environmental cues, and highlight how filamentcharacteristics of Bordetella T3SS injectisome change in response to differentenvironments.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EH22_008%2F0004597" target="_blank" >EH22_008/0004597: Mluvíme s mikroby - porozumění mikrobiálním interakcím v konceptu One Health</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

mBio

ISSN

2161-2129

e-ISSN

2150-7511

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

"nestrankovano"

Kód UT WoS článku

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EID výsledku v databázi Scopus

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